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Journal of Clinical Microbiology, April 2006, p. 1428-1434, Vol. 44, No. 4
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.4.1428-1434.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Oncogenic Human Papillomavirus (HPV) Type Distribution and HPV Type 16 E6 Variants in Two Spanish Population Groups with Different Levels of HPV Infection Risk

M. Ortiz,1* M. Torres,1 L. Muñoz,1 E. Fernández-García,2 J. Canals,3 A. I. Cabornero,1 E. Aguilar,4 J. Ballesteros,5 J. del Amo,6 and A. García-Sáiz1

Servicio de Diagnóstico y Referencia de Retrovirus y Papilomavirus, CNM, Instituto de Salud Carlos III, Majadahonda,1 Centro Sanitario Sandoval, Madrid,5 Centro de Información y Prevención del SIDA,2 Centro de Planificación Familiar No. II, Alicante,3 Centro Madrid Salud Ciudad Lineal, Servicio de Prevención y Promoción de Salud, Instituto de Salud Pública Madrid-Salud, Ayuntamiento de Madrid, Madrid,4 Departamento de Salud Pública, Universidad Miguel Hernández, Alicante, Spain6

Received 23 November 2005/ Returned for modification 9 January 2006/ Accepted 14 February 2006

The aim of this study is to determine oncogenic human papillomavirus (HPV) types and HPV type 16 (HPV16) variant distribution in two Spanish population groups, commercial sex workers and imprisoned women (CSW/IPW) and the general population. A multicenter cross-sectional study of 1,889 women from five clinical settings in two Spanish cities was conducted from May to November 2004. Oncogenic HPV infection was tested by an Hybrid Capture II (HC2) test, and positive samples were genotyped by direct sequencing using three different primer sets in L1 (MY09/11 and GP5+/GP6+) and E6/E7. HPV16 variants were identified by sequencing the E6, E2, and L1 regions. Four hundred twenty-five samples were positive for the HC2 test, 31.5% from CSW/IPW and 10.7% from the general population. HPV16 was the most frequent type. Distinct profiles of oncogenic HPV type prevalence were observed across the two populations. In order of decreasing frequency, HPV types 16, 31, 58, 66, 56, and 18 were most frequent in CSW/IPW women, and types 16, 31, 52, 68, 51, and 53 were most frequent in the general population. We analyzed HPV16 intratype variants, and a large majority (78.7%) belonged to the European lineage. AA variants were detected in 16.0% of cases. African variants belonging to classes Af1 (4.0%) and Af2 (1.3%) were detected. Different HPV types and HPV16 intratype variants are involved in oncogenic HPV infections in our population. These results suggest that HPV type distribution differs in CSW/IPW women and in the general population, although further analysis is necessary.

* Corresponding author. Mailing address: Servicio de Diagnóstico y Referencia de Retrovirus y Papilomavirus, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra. Majadahonda-Pozuelo Km 2, 28220, Majadahonda-Madrid, Spain. Phone: 34-91-822-36-26. Fax: 34-91-509-79-48. E-mail: mortiz{at}isciii.es.

Journal of Clinical Microbiology, April 2006, p. 1428-1434, Vol. 44, No. 4
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.4.1428-1434.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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