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Journal of Clinical Microbiology, April 2006, p. 1530-1535, Vol. 44, No. 4
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.4.1530-1535.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Invasive Haemophilus influenzae in Manitoba, Canada, in the Postvaccination Era

Raymond S. W. Tsang,1* Samira Mubareka,2 Michelle L. Sill,1 John Wylie,3 Stuart Skinner,2 and Dennis K. S. Law1

Laboratory of Vaccine Preventable Bacterial Diseases, National Microbiology Laboratory, Public Health Agency of Canada,1 Department of Medical Microbiology and Infectious Diseases, University of Manitoba,2 Cadham Provincial Public Health Laboratory, Manitoba Health, Winnipeg, Manitoba, Canada3

Received 7 December 2005/ Returned for modification 18 January 2006/ Accepted 24 January 2006

Fifty-two Haemophilus influenzae isolates from patients with invasive disease in the province of Manitoba, Canada, were examined for serotype, biotype, genotype, and antibiotic susceptibility. Half of the 52 isolates were found to be serotype a, and 38.5% (20 isolates) were found to be nonserotypeable (NST). There were only three serotype b strains and one each for serotypes c, d, and f. All 26 serotype a isolates belonged to biotype II and demonstrated identical or highly similar DNA fingerprints by pulsed-field gel electrophoresis. An analysis of these isolates by multilocus sequence typing showed that they belong to the clonal complex ST-23. While 69% (18 of 26) of the serotype a cases were found in males, only 9 (45%) of the 20 patients with NST isolates were males. Twenty (77%) of the 26 serotype a isolates were from patients who were ≤24 months old. Twelve (63%) of the NST isolates were from adult or adolescent patients. In contrast to the clonal nature of serotype a isolates, the 20 NST isolates were found to belong to 18 different sequence types. Most of these 18 different sequence types were unrelated to each other, with the exception of 7 sequence types grouped into three clonal groups. Two (6.25%) out of 32 serotypeable isolates (1 serotype a and 1 serotype b) and 6 (30%) of 20 NST isolates were resistant to ampicillin due to ß-lactamase production. These results suggest a change in the epidemiology of H. influenzae disease, with the majority of invasive H. influenzae isolates being associated with serotype a and NST strains.

* Corresponding author. Mailing address: Vaccine Preventable Bacterial Disease Section, National Microbiology Laboratory, 1015 Arlington Street, Winnipeg, Manitoba, Canada R3E 3R2. Phone: (204) 789-6020. Fax: (204) 789-2018. E-mail: raymond_tsang{at}phac-aspc.gc.ca.

Journal of Clinical Microbiology, April 2006, p. 1530-1535, Vol. 44, No. 4
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.4.1530-1535.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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