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Journal of Clinical Microbiology, April 2006, p. 1567-1570, Vol. 44, No. 4
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.4.1567-1570.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Rapid Identification of Enterococcus hirae and Enterococcus durans by PCR and Detection of a Homologue of the E. hirae mur-2 Gene in E. durans

Cesar A. Arias,1,2,5 Beatriz Robredo,4 Kavindra V. Singh,1,2 Carmen Torres,4 Diana Panesso,5 and Barbara E. Murray1,2,3*

Center for the Study of Emerging and Reemerging Pathogens,1 Division of Infectious Diseases, Department of Internal Medicine,2 Department of Microbiology and Molecular Genetics, The University of Texas Medical School at Houston, Houston, Texas 77030,3 Area de Bioquímica y Biología Molecular, Universidad de La Rioja, 26006 Logroño, Spain,4 Bacterial Molecular Genetics Unit, Universidad El Bosque, Transv 9aA Bis No. 133-25, Bogotá, D.C., Colombia5

Received 19 November 2005/ Returned for modification 23 January 2006/ Accepted 9 February 2006

During an evaluation of PCR for identification of isolates of Enterococcus hirae, a homologue with 82% identity to E. hirae mur-2 was identified in Enterococcus durans and was named mur-2ed. PCR using primers for two genes (copY and murG) of E. hirae strains showed amplification with E. hirae strains only. PCR (under high-stringency conditions) with primers for the mur-2ed gene gave the expected amplification product only with E. durans strains. A combination of murG and mur-2ed primers in a multiplex PCR assay differentiated E. hirae from E. durans in all cases. PCR using these primers appears to be a rapid alternative for identification of E. hirae and E. durans isolates.

* Corresponding author. Mailing address: 6431 Fannin St. 2.112 MSB, University of Texas Medical School, Houston, TX 77030. Phone: (713) 500-6745. Fax: (713) 500-6766. E-mail: barbara.e.murray{at}uth.tmc.edu.

Journal of Clinical Microbiology, April 2006, p. 1567-1570, Vol. 44, No. 4
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.4.1567-1570.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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