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Journal of Clinical Microbiology, May 2006, p. 1619-1624, Vol. 44, No. 5
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.5.1619-1624.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Comparison of a Conventional Antimicrobial Susceptibility Assay to an Oligonucleotide Chip System for Detection of Drug Resistance in Mycobacterium tuberculosis Isolates

Heekyung Park,^1, Eun Ju Song,^2, Eun Sil Song,¹ Eun Yup Lee,² Cheol Min Kim,^1,3,9 Seok Hoon Jeong,⁴ Jeong Hwan Shin,⁵ Joseph Jeong,⁶ Sunjoo Kim,⁷ Young Kil Park,⁸ Gill-Han Bai,⁸ and Chulhun L. Chang^2,8,9*

Institute for Genomic Medicine, GeneIn Co., Ltd., Busan, Korea; Departments of,¹ Laboratory Medicine,² Biochemistry, College of Medicine, Pusan National University, Busan, Korea,³ Department of Laboratory Medicine, College of Medicine, Kosin University, Busan, Korea,⁴ Department of Laboratory Medicine, College of Medicine, Inje University, Busan, Korea,⁵ Department of Laboratory Medicine, College of Medicine, Ulsan University, Ulsan, Korea,⁶ Department of Laboratory Medicine, College of Medicine, Gyeongsang National University, Jinju, Korea,⁷ Korean Institute of Tuberculosis, The Korean National Tuberculosis Association, Seoul, Korea,⁸ Medical Research Institute, Pusan National University, Busan, Korea⁹

Received 5 September 2005/ Returned for modification 8 November 2005/ Accepted 7 March 2006

An oligonucleotide chip (Combichip Mycobacteria chip) detecting specific mutations in the rpoB, katG, and inhA genes of Mycobacterium tuberculosis was compared with conventional antimicrobial susceptibility results. The probes detecting drug resistance were as follows: 7 wild-type and 13 mutant probes for rifampin and 2 wild-type and 3 mutant probes for isoniazid. Target DNA of M. tuberculosis was amplified by PCR, followed by hybridization and scanning. Direct sequencing was performed to verify the results of the oligonucleotide chip. One-hundred seven of 115 rifampin-resistant strains (93%) had mutations in the rpoB gene. Eighty-five of 119 isoniazid-resistant strains (71%) had mutations in the katG gene or inhA gene. The diagnostic oligonucleotide chip with mutation-specific probes is a reliable and useful tool for the rapid and accurate diagnosis of resistance against rifampin and isoniazid in M. tuberculosis isolates.

H.P. and E.J.S. participated equally in this study.

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