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Journal of Clinical Microbiology, May 2006, p. 1788-1791, Vol. 44, No. 5
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.5.1788-1791.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Comparative Evaluation of the Performance of the Abbott Real-Time Human Immunodeficiency Virus Type 1 (HIV-1) Assay for Measurement of HIV-1 Plasma Viral Load following Automated Specimen Preparation

Department of Virology, Royal Free Hospital and Royal Free and University College Medical School, London, United Kingdom

Received 27 October 2005/ Returned for modification 7 December 2005/ Accepted 13 February 2006

The performance of the new Abbott real-time human immunodeficiency virus type 1 (HIV-1) assay for HIV-1 RNA load determination in plasma was compared to that of the Abbott LCx HIV-1 RNA quantitative assay following automated RNA isolation by the Abbott m1000 extractor. The measured viral loads of 89 clinical specimens differed by mean 0.19 log₁₀ copies/ml (95% confidence interval, 0.12 to 0.26 log₁₀ copies/ml). Although the difference in viral load determinations was positively skewed in favor of the LCx assay, it did not reach statistical significance (P = 0.42). Results were linearly associated (R² = 0.94) and strongly correlated (R = 0.96). Good performance was observed with HIV-1 subtypes other than B and circulating recombinant forms, although results obtained with two subtype G specimens and one H specimen showed a more substantial difference.

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