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Journal of Clinical Microbiology, May 2006, p. 1836-1838, Vol. 44, No. 5
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.5.1836-1838.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Rapid Differentiation between Members of the Anginosus Group and Streptococcus dysgalactiae subsp. equisimilis within Beta-Hemolytic Group C and G Streptococci by PCR

Liang-Chun Liu,1 Jui-Chang Tsai,2,4 Po-Ren Hsueh,3 and Lee-Jene Teng1,3*

Department of Clinical Laboratory Sciences and Medical Biotechnology,1 Center for Optoelectronic Biomedicine, National Taiwan University College of Medicine,2 Department of Laboratory Medicine,3 Division of Neurosurgery, Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan4

Received 6 January 2006/ Accepted 14 February 2006

Based on a pair of primers developed initially for differentiating the anginosus group from other viridans streptococci, the PCR reported here can also differentiate between members of the anginosus group and Streptococcus dysgalactiae subsp. equisimilis among beta-hemolytic group C and G streptococci. The resulting 742-bp PCR product was specific for members of the anginosus group, although a smaller, nonspecific product (361 bp) was generated from S. dysgalactiae subsp. equisimilis. Restriction digestion of the amplicon with XbaI and BsmI further differentiated Streptococcus anginosus from Streptococcus constellatus within the anginosus group.

* Corresponding author. Mailing address: Department of Clinical Laboratory Sciences and Medical Biotechnology, National Taiwan University College of Medicine, 1 Chang-Te Street, Taipei 100, Taiwan. Phone: 886-2-23123456, ext. 6918. Fax: 886-2-23711574. E-mail: ljteng{at}ha.mc.ntu.edu.tw.

Journal of Clinical Microbiology, May 2006, p. 1836-1838, Vol. 44, No. 5
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.5.1836-1838.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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