This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Liu, L.-C. Articles by Teng, L.-J. Search for Related Content PubMed PubMed Citation Articles by Liu, L.-C. Articles by Teng, L.-J.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 2006, p. 1836-1838, Vol. 44, No. 5
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.5.1836-1838.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Rapid Differentiation between Members of the Anginosus Group and Streptococcus dysgalactiae subsp. equisimilis within Beta-Hemolytic Group C and G Streptococci by PCR

Department of Clinical Laboratory Sciences and Medical Biotechnology,¹ Center for Optoelectronic Biomedicine, National Taiwan University College of Medicine,² Department of Laboratory Medicine,³ Division of Neurosurgery, Department of Surgery, National Taiwan University Hospital, Taipei, Taiwan⁴

Received 6 January 2006/ Accepted 14 February 2006

Based on a pair of primers developed initially for differentiating the anginosus group from other viridans streptococci, the PCR reported here can also differentiate between members of the anginosus group and Streptococcus dysgalactiae subsp. equisimilis among beta-hemolytic group C and G streptococci. The resulting 742-bp PCR product was specific for members of the anginosus group, although a smaller, nonspecific product (361 bp) was generated from S. dysgalactiae subsp. equisimilis. Restriction digestion of the amplicon with XbaI and BsmI further differentiated Streptococcus anginosus from Streptococcus constellatus within the anginosus group.