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Journal of Clinical Microbiology, May 2006, p. 1859-1862, Vol. 44, No. 5
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.5.1859-1862.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Novel Approach to Designing Primers for Identification and Distinction of the Human Pathogenic Fungi Coccidioides immitis and Coccidioides posadasii by PCR Amplification

Takashi Umeyama,1 Ayako Sano,2 Katsuhiko Kamei,2 Masakazu Niimi,1 Kazuko Nishimura,2 and Yoshimasa Uehara1*

Department of Bioactive Molecules, National Institute of Infectious Diseases, Tokyo 162-8640,1 Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba 260-8673, Japan2

Received 10 January 2006/ Returned for modification 28 February 2006/ Accepted 3 March 2006

We developed a pair of primers that specifically identifies Coccidioides species, etiologic agents of the human fungal disease coccidioidomycosis. These primers could be used for distinguishing Coccidioides immitis and Coccidioides posadasii by simply comparing the amplicon sizes on an agarose gel.

* Corresponding author. Mailing address: Department of Bioactive Molecules, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8640, Japan. Phone: 81 3 5285-1111. Fax: 81 3 5285-1175. E-mail: yuehara{at}nih.go.jp.

Journal of Clinical Microbiology, May 2006, p. 1859-1862, Vol. 44, No. 5
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.5.1859-1862.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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