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Journal of Clinical Microbiology, May 2006, p. 1884-1886, Vol. 44, No. 5
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.5.1884-1886.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

PCR Amplification of the IS6110 Insertion Element of Mycobacterium tuberculosis in Fecal Samples from Patients with Intestinal Tuberculosis

Ramadass Balamurugan,1 Subramanian Venkataraman,1 K. R. John,2 and Balakrishnan S. Ramakrishna1*

Departments of Gastrointestinal Sciences,1 Community Health, Christian Medical College, Vellore, India2

Received 29 November 2005/ Returned for modification 26 January 2006/ Accepted 7 March 2006

PCR amplification of insertion element IS6110 of Mycobacterium tuberculosis in fecal samples was evaluated in the diagnosis of intestinal tuberculosis (ITB). The numbers of samples that tested positive by PCR with SalI digestion were 16/18 untreated-ITB samples, 0/8 treated-ITB samples, 12/14 smear-positive pulmonary tuberculosis samples, and 0/30 control samples. The sensitivity, specificity, positive predictive value, and negative predictive value of fecal PCR were 88.8%, 100%, 100%, and 93.7%, respectively.

* Corresponding author. Mailing address: Department of Gastrointestinal Sciences, Christian Medical College, Ida Scudder Road, Vellore 632004, Tamil Nadu, India. Phone: 91 416 2282052. Fax: 91 416 2232035. E-mail: rama{at}cmcvellore.ac.in.

Journal of Clinical Microbiology, May 2006, p. 1884-1886, Vol. 44, No. 5
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.5.1884-1886.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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