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Journal of Clinical Microbiology, May 2006, p. 1892-1895, Vol. 44, No. 5
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.5.1892-1895.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Rapid Staphylococcus aureus agr Type Determination by a Novel Multiplex Real-Time Quantitative PCR Assay

Patrice Francois,1* Thibaud Koessler,1 Antoine Huyghe,1 Stephan Harbarth,2 Manuela Bento,1 Daniel Lew,1,3 Jérôme Etienne,4 Didier Pittet,2 and Jacques Schrenzel1,3

Genomic Research Laboratory,1 Infection Control Program,2 Clinical Microbiology Laboratory, University of Geneva Hospitals (HUG), Service of Infectious Diseases, CH-1211 Geneva 14, Switzerland,3 Centre National de Référence des Staphylocoques, INSERM E0230, Lyon, France4

Received 17 October 2005/ Returned for modification 29 November 2005/ Accepted 10 March 2006

The accessory gene regulator (agr) is a crucial regulatory component of Staphylococcus aureus involved in the control of bacterial virulence factor expression. We developed a real-time multiplex quantitative PCR assay for the rapid determination of S. aureus agr type. This assay represents a rapid and affordable alternative to sequence-based strategies for assessing relevant epidemiological information.

* Corresponding author. Mailing address: University of Geneva Hospitals, Service of Infectious Diseases/Genomic Research Laboratory, CH-1211 Geneva 14, Switzerland. Phone: (41) 22-372-9338. Fax: (41) 22-372-9830. E-mail: patrice.francois{at}genomic.ch.

Journal of Clinical Microbiology, May 2006, p. 1892-1895, Vol. 44, No. 5
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.5.1892-1895.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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