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Journal of Clinical Microbiology, May 2006, p. 1896-1898, Vol. 44, No. 5
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.5.1896-1898.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Application of Minimal Sequence Quality Values Prevents Misidentification of the blaSHV Type in Single Bacterial Isolates Carrying Different SHV Extended-Spectrum ß-Lactamase Genes

Nashwan al Naiemi, Kim Schipper, Birgitta Duim, and Aldert Bart*

Department of Medical Microbiology, Academic Medical Center, Amsterdam, The Netherlands

Received 14 February 2006/ Accepted 28 February 2006

Nucleotide sequencing is the standard molecular method for determination of the ß-lactamase gene present in an isolate. Using minimal sequence quality values prevents misidentification of blaSHV genes, as illustrated by three strains of three different species that each contained two different blaSHV alleles, SHV-2 and SHV-12.

* Corresponding author. Mailing address: Department of Medical Microbiology, Academic Medical Center, L1-116, P.O. Box 22660, 1100 DD Amsterdam, The Netherlands. Phone: 31 20 5664863. Fax: 31 20 6979271. E-mail: a.bart{at}amc.uva.nl.

Journal of Clinical Microbiology, May 2006, p. 1896-1898, Vol. 44, No. 5
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.5.1896-1898.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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