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Journal of Clinical Microbiology, May 2006, p. 1899-1902, Vol. 44, No. 5
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.5.1899-1902.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Development and Evaluation of a Loop-Mediated Isothermal Amplification Method for Rapid Diagnosis of Bordetella pertussis Infection

Kazunari Kamachi,1* Hiromi Toyoizumi-Ajisaka,1 Kohei Toda,2 Sann Chan Soeung,3 Svay Sarath,3 Ya Nareth,3 Yoshinobu Horiuchi,1 Kazunobu Kojima,4 Motohide Takahashi,1 and Yoshichika Arakawa1

Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo, Japan,1 World Health Organization Representative Office,2 Department of National Immunization Program, Ministry of Health, Phnom Penh, Cambodia,3 World Health Organization Western Pacific Regional Office, Manila, Philippines4

Received 22 December 2005/ Returned for modification 29 January 2006/ Accepted 7 March 2006

We developed a loop-mediated isothermal amplification (LAMP) method to detect Bordetella pertussis infection. This LAMP assay detected B. pertussis with high sensitivity, but not other Bordetella species. Among nasopharyngeal swab samples from subjects with suspected pertussis, LAMP results showed a high level of agreement with results of conventional PCR. This method is a rapid, sensitive, and specific method for diagnosis of B. pertussis infection even in clinical laboratories with no specific equipment.

* Corresponding author. Mailing address: Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, 4-7-1 Gakuen, Musashimurayma-city, Tokyo 208-0011, Japan. Phone: 81-42-561-0771. Fax: 81-42-565-3315. E-mail: kamachi{at}nih.go.jp.

Journal of Clinical Microbiology, May 2006, p. 1899-1902, Vol. 44, No. 5
0095-1137/06/$08.00+0     doi:10.1128/JCM.44.5.1899-1902.2006
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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