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Journal of Clinical Microbiology, June 2006, p. 1971-1976, Vol. 44, No. 6
0095-1137/06/$08.00+0     doi:10.1128/JCM.00062-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Identification of Extended-Spectrum, AmpC, and Carbapenem- Hydrolyzing ß-Lactamases in Escherichia coli and Klebsiella pneumoniae by Disk Tests

George A. Jacoby,* Kelley E. Walsh, and Victoria J. Walker

Lahey Clinic, Burlington, Massachusetts

Received 11 January 2006/ Returned for modification 16 March 2006/ Accepted 27 March 2006

Antibiotic disks with and without clavulanic acid, 3-aminophenylboronic acid, or EDTA were tested with a set of 55 Klebsiella pneumoniae and Escherichia coli strains producing well-characterized extended-spectrum, AmpC, or carbapenem-hydrolyzing ß-lactamases. A relatively simple scheme was devised for distinguishing ß-lactamase types in clinical isolates with or without intact outer membrane porins.

* Corresponding author. Mailing address: Lahey Clinic, 41 Mall Road, Burlington, MA 01895. Phone: (781) 744-2928. Fax: (781) 744-5486. E-mail: george.a.jacoby{at}lahey.org.

Journal of Clinical Microbiology, June 2006, p. 1971-1976, Vol. 44, No. 6
0095-1137/06/$08.00+0     doi:10.1128/JCM.00062-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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