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Journal of Clinical Microbiology, June 2006, p. 2153-2157, Vol. 44, No. 6
0095-1137/06/$08.00+0     doi:10.1128/JCM.02540-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Microsatellite Analysis of Three Phylogenetic Species of Paracoccidioides brasiliensis{dagger}

Daniel R. Matute,1,{ddagger} Victoria E. Sepulveda,1,{ddagger} Lina M. Quesada,2 Gustavo H. Goldman,3 John W. Taylor,4 Angela Restrepo,1,5 and Juan G. McEwen1,6*

Corporación para Investigaciones Biológicas (CIB), Medellín, Colombia,1 Laboratorio de Micología y Fitopatología Uniandes, Universidad de los Andes, Bogotá, Colombia,2 Faculdade de Ciências Farmacêuticas de Riberão Preto, Universidade de São Paulo, São Paulo, Brazil,3 Department of Plant and Microbial Biology, 111 Koshland Hall, University of California, Berkeley, California 94720-3102,4 Facultad de Medicina, Universidad Pontificia Bolivariana, Medellín, Colombia,5 Facultad de Medicina, Universidad de Antioquia, Medellín, Colombia6

Received 6 December 2005/ Returned for modification 27 January 2006/ Accepted 27 March 2006

Paracoccidioides brasiliensis is the etiological agent of paracoccidioidomycosis, an important human systemic mycosis in Latin America. Recently, the existence of three different phylogenetic species (S1, PS2, and PS3) of P. brasiliensis was demonstrated. Despite being genetically isolated, all three species were capable of inducing disease in both humans and animals, although lower virulence has been found with the PS2 species. The available molecular methods developed to characterize and type strains have not been useful for assigning isolates to the described species, creating the need for molecular markers capable of distinguishing genetically isolated groups. Here, we describe a PCR and sequencing-based microsatellite marker system that is stable, easy to assay, adaptable to large series of isolates, and discriminatory enough to be used as a typing system in identifying the three proposed species of P. brasiliensis. In addition, this system provides an unambiguous tool for strain discrimination between two (S1 and PS2) of the three phylogenetic species.

* Corresponding author. Mailing address: Carrera 72A 78B-141, Medellín, Colombia. Phone: (574)-441-08 55. Fax: (574)-441-55 14. E-mail: mcewen{at}epm.net.co.

{dagger} Supplemental material for this article may be found at http://jcm.asm.org/.

{ddagger} D.R.M. and V.E.S. contributed equally to this work.

Journal of Clinical Microbiology, June 2006, p. 2153-2157, Vol. 44, No. 6
0095-1137/06/$08.00+0     doi:10.1128/JCM.02540-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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