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Journal of Clinical Microbiology, June 2006, p. 2254-2257, Vol. 44, No. 6
0095-1137/06/$08.00+0     doi:10.1128/JCM.00690-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Blinded Comparison of Repetitive-Sequence PCR and Multilocus Sequence Typing for Genotyping Methicillin-Resistant Staphylococcus aureus Isolates from a Children's Hospital in St. Louis, Missouri

Robert S. Liao,1,{dagger} Gregory A. Storch,2 Richard S. Buller,2 Rachel C. Orscheln,2 Elaine R. Mardis,3 Jon R. Armstrong,3 and W. Michael Dunne Jr.1*

Departments of Pathology and Immunology,1 Pediatrics,2 the Genome Sequencing Center, Washington University School of Medicine, St. Louis, Missouri 631103

Received 31 March 2006/ Accepted 10 April 2006

We performed a blinded study to compare repetitive-sequence PCR and multilocus sequence typing for genotyping hospital- and community-acquired methicillin-resistant Staphylococcus aureus (MRSA). The MRSA strains that were sequence type 8 (ST8), staphylococcal cassette chromosome mec (SCCmec) type IV, and Panton-Valentine leukocidin-positive clustered separately from those that were ST5 and SCCmec type II.

* Corresponding author. Mailing address: Division of Laboratory Medicine, Department of Pathology and Immunology, Washington University School of Medicine, 660 South Euclid Avenue, Campus Box 8118, St. Louis, MO 63110. Phone: (314) 362-2998. Fax: (314) 362-1461. E-mail: dunne{at}wustl.edu.

{dagger} Present address: Pathology and Molecular Medicine, Kingston General Hospital, Douglas 3, Room 315, 76 Stuart Street, Kingston, Ontario K7L 2V7, Canada.

Journal of Clinical Microbiology, June 2006, p. 2254-2257, Vol. 44, No. 6
0095-1137/06/$08.00+0     doi:10.1128/JCM.00690-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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Copyright © 2006 by the American Society for Microbiology. All rights reserved.