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Journal of Clinical Microbiology, June 2006, p. 2258-2261, Vol. 44, No. 6
0095-1137/06/$08.00+0     doi:10.1128/JCM.00530-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Comparison of a Stool Antigen Detection Kit and PCR for Diagnosis of Entamoeba histolytica and Entamoeba dispar Infections in Asymptomatic Cyst Passers in Iran

Shahram Solaymani-Mohammadi,1,3 Mostafa Rezaian,1 Zahra Babaei,1 Azam Rajabpour,2 Ahmad R. Meamar,1 Ahmad A. Pourbabai,2 and William A. Petri Jr.3*

Division of Intestinal and Genital Protozoal Diseases, Department of Medical Parasitology and Mycology, School of Public Health and Institute of Public Health Research, Tehran University of Medical Sciences, 14155 Tehran 6446, Iran,1 Department of Microbiology, School of Science, Qom Azad University, Qom, Iran,2 Division of Infectious Diseases and International Health, Department of Internal Medicine, University of Virginia Health System, Charlottesville, Virginia 22908-13403

Received 10 March 2006/ Accepted 21 March 2006

The present study was conducted to compare stool antigen detection with PCR for the diagnosis of Entamoeba sp. infection in asymptomatic cyst passers from Iran. Entamoeba dispar and, in one case, E. moshkovskii were the Entamoeba spp. found in the amebic cyst passers. There was a 100% correlation between the results from the TechLab E. histolytica II stool antigen kit and those from nested PCR. We concluded that E. dispar is much more common in asymptomatic cyst passers in Iran and that antigen detection and PCR are comparable diagnostic modalities.

* Corresponding author. Mailing address: Division of Infectious Diseases and International Health, P.O. Box 801340, Rm. 2115, MR4 Building, University of Virginia Health System, Charlottesville, VA 22908-1340. Phone: (434) 924-5621. Fax: (434) 924-0075. E-mail: wap3g{at}virginia.edu.

Journal of Clinical Microbiology, June 2006, p. 2258-2261, Vol. 44, No. 6
0095-1137/06/$08.00+0     doi:10.1128/JCM.00530-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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