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Journal of Clinical Microbiology, June 2006, p. 2262-2264, Vol. 44, No. 6
0095-1137/06/$08.00+0     doi:10.1128/JCM.00635-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Comparison of the BACTEC 9240 and BacT/Alert Blood Culture Systems for Detection of Bacterial Contamination in Platelet Concentrates

Stefan Riedel, Gregory Siwek,{dagger} Susan E. Beekmann, Sandra S. Richter, Thomas Raife, and Gary V. Doern*

Divisions of Clinical Microbiology and Transfusion Medicine, Department of Pathology, University of Iowa College of Medicine, Iowa City, Iowa 52242

Received 24 March 2006/ Returned for modification 4 April 2006/ Accepted 6 April 2006

The BACTEC 9240 blood culture system with a standard aerobic medium, Plus Aerobic/F, was compared to the BacT/Alert system with a platelet-specific medium, BPA, as a means for detecting bacterial contamination of platelet preparations. One hundred thirteen platelet units seeded with low levels of different bacteria were examined with both systems. In 93 instances, growth was detected first in the BACTEC system; in 12 cases, growth registered first in the BacT/Alert system. Among all comparisons, growth was detected, on average, 1.7 h sooner with the BACTEC system. The differences in length of time to detection were statistically significant.

* Corresponding author. Mailing address: Clinical Microbiology Laboratories, University of Iowa Hospital, C606-GH Boyd Tower, Iowa City, IA 52242. Phone: (319) 356-8616. Fax: (319) 356-4916. E-mail: gary-doern{at}uiowa.edu.

{dagger} Present address: Health Partners Specialty Center, Infectious Diseases, 410 Phalen Boulevard, St. Paul, MN 55101.

Journal of Clinical Microbiology, June 2006, p. 2262-2264, Vol. 44, No. 6
0095-1137/06/$08.00+0     doi:10.1128/JCM.00635-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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