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Journal of Clinical Microbiology, June 2006, p. 2276-2279, Vol. 44, No. 6
0095-1137/06/$08.00+0     doi:10.1128/JCM.02017-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Nongastric Helicobacter Species Detected in the Intestinal Tract of Children

Li Zhang,1 Andrew Day,2 Gavin McKenzie,3 and Hazel Mitchell1*

School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia,1 Department of Gastroenterology, Sydney Children's Hospital, School of Women's and Children's Health, University of New South Wales, Sydney, Australia,2 Histology and Microscopy Unit, School of Medical Sciences, University of New South Wales, Sydney, Australia3

Received 26 September 2005/ Returned for modification 18 January 2006/ Accepted 10 March 2006

The presence of members of the family Helicobacteraceae in the colon of children was investigated using PCR, denaturing gradient gel electrophoresis, and fluorescent in situ hybridization. The rate of detection of species belonging to the Helicobacteraceae family in children with inflammatory bowel disease (92%) and irritable bowel syndrome (100%) was found to be significantly higher than that in healthy children (25%) (P < 0.05).


* Corresponding author. Mailing address: School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia. Phone: 61 (2) 9385 2040. Fax: 61 (2) 9385 1591. E-mail: h.mitchell{at}unsw.edu.au.


Journal of Clinical Microbiology, June 2006, p. 2276-2279, Vol. 44, No. 6
0095-1137/06/$08.00+0     doi:10.1128/JCM.02017-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.




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