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Journal of Clinical Microbiology, June 2006, p. 2280-2282, Vol. 44, No. 6
0095-1137/06/$08.00+0     doi:10.1128/JCM.02187-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Evaluation of a Real-Time Nucleic Acid Sequence-Based Amplification Assay Using Molecular Beacons for Detection of Human Immunodeficiency Virus Type 1

D. R. McClernon,* C. Vavro, and M. St. Clair

Clinical Virology Department, GlaxoSmithKline, Research Triangle Park, North Carolina

Received 18 October 2005/ Returned for modification 15 December 2005/ Accepted 15 March 2006

We evaluated the performance characteristics of a new, real-time nucleic acid sequence-based amplification (NASBA) assay that incorporates molecular beacon technology for detection of human immunodeficiency virus type 1 (HIV-1). The quantitative results were comparable to those obtained with three leading commercially available assays. The analytical sensitivity was 37 IU/ml. The NASBA assay detected clinically relevant recombinant viruses and all group M HIV-1 subtypes.

* Corresponding author. Mailing address: Clinical Virology Department, GlaxoSmithKline, P.O. Box 13398, Research Triangle Park, NC 27709-3398. Phone: (919) 483-9425. Fax: (919) 315-0068. E-mail: daniel.r.mcclernon{at}gsk.com.

Journal of Clinical Microbiology, June 2006, p. 2280-2282, Vol. 44, No. 6
0095-1137/06/$08.00+0     doi:10.1128/JCM.02187-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Zhao, Y., Park, S., Kreiswirth, B. N., Ginocchio, C. C., Veyret, R., Laayoun, A., Troesch, A., Perlin, D. S. (2009). Rapid Real-Time Nucleic Acid Sequence-Based Amplification-Molecular Beacon Platform To Detect Fungal and Bacterial Bloodstream Infections. J. Clin. Microbiol. 47: 2067-2078 [Abstract] [Full Text]  
  • McClernon, D. R., Ramsey, E., StClair, M. (2007). Magnetic Silica Extraction for Low-Viremia Human Immunodeficiency Virus Type 1 Genotyping. J. Clin. Microbiol. 45: 572-574 [Abstract] [Full Text]  


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