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Journal of Clinical Microbiology, July 2006, p. 2354-2358, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.00171-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Detection of Multidrug-Resistant Salmonella enterica Serovar Typhimurium Phage Types DT102, DT104, and U302 by Multiplex PCR

Cheng-Hsun Chiu,1,2 Lin-Hui Su,2,3 Chi-Hong Chu,4 Mei-Hwei Wang,1 Chia-Ming Yeh,5 Francois-Xavier Weill,6 and Chishih Chu5*

Department of Pediatrics, Chang Gung Children's Hospital, Taoyuan,1 Chang Gung University College of Medicine, Taoyuan,2 Department of Clinical Pathology, Chang Gung Memorial Hospital, Taoyuan,3 Department of General Surgery, Tri-Service Hospital and National Defense Medical Center, Taipei,4 Department of Applied Microbiology, National Chiayi University, Chiayi, Taiwan,5 Centre National de Référence des Salmonella, Institut Pasteur, Paris, France6

Received 26 January 2006/ Returned for modification 7 March 2006/ Accepted 29 April 2006

Salmonella enterica serovar Typhimurium is a common cause of nontyphoidal salmonellosis in humans and animals. Multidrug-resistant serovar Typhimurium phage type DT104, which emerged in the 1990s, has become widely distributed in many countries. A total of 104 clinical isolates of Salmonella serogroup B were collected from three major hospitals in Taiwan during 1997 to 2003 and were examined by a multiplex PCR targeting the resistance genes and the spv gene of the virulence plasmid. A total of 51 isolates (49%) were resistant to all drugs (ACSSuT [resistance to ampicillin, chloramphenicol, streptomycin, sulfonamide, and tetracycline]), and all contained a 1.25-kb PCR fragment of integron that is part of the 43-kb Salmonella genomic island 1 (SGI1). The second group was resistant to SSu (28%), and the third was susceptible to all five drugs (13%). Fifty-nine isolates were serotyped to be serovar Typhimurium by the tube agglutination method using H antisera. The virulence plasmid was found in 54 (91.5%) of the 59 serovar Typhimurium isolates. A majority (94.1%) of the Salmonella serogroup B isolates with the ACSSuT resistance pattern harbored a virulence plasmid. Phage typing identified three major phage types: DT104, DT120, and U302. Analysis of the isolates by pulsed-field gel electrophoresis showed six genotypes. We found two genotypes in DT104 strains, two in DT120, and the other two in U302. The presence of a monophasic serovar (4,5,12:i:–) has added difficulty in the determination of the serovars of multidrug-resistant Salmonella serogroup B isolates. Nevertheless, the multiplex PCR devised in the present study appears to be efficient and useful in the rapid identification of ACSSuT-type serovar Typhimurium with SGI1, irrespective of their phage types.

* Corresponding author. Mailing address: Department of Applied Microbiology, National Chiayi University, 300 Syuefu Road, Chiayi 60004, Taiwan. Phone: 886-5-2717898. Fax: 886-5-2717831. E-mail: cschu{at}mail.ncyu.edu.tw.

Journal of Clinical Microbiology, July 2006, p. 2354-2358, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.00171-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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