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Journal of Clinical Microbiology, July 2006, p. 2434-2441, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.00397-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Molecular Epidemiologic Identification of Escherichia coli Genes That Are Potentially Involved in Movement of the Organism from the Intestinal Tract to the Vagina and Bladder

Jingping Xie,1,2 Betsy Foxman,1 Lixin Zhang,1 and Carl F. Marrs1*

Department of Epidemiology, University of Michigan School of Public Health, Ann Arbor, Michigan,1 Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, Michigan2

Received 22 February 2006/ Returned for modification 10 April 2006/ Accepted 10 May 2006

A first step in urinary tract infection (UTI) pathogenesis in the otherwise healthy host is the movement of uropathogenic Escherichia coli from the intestinal tract to the urinary tract. We conducted a genomic subtraction to isolate genetic regions associated with this movement. A representative UTI isolate present in the rectum, vagina, and bladder of a woman with UTI was chosen as the tester; the driver was a phylogenetically distant rectal isolate (based on pulsed-field gel electrophoresis analysis) with a profile of uropathogenic virulence genes similar to that of the tester. Tester-specific regions identified by the subtraction were screened, using DNA dot blot hybridization, against a collection of 88 uropathogens isolated from the rectum, urine, and/or vagina of women with UTIs and 54 E. coli isolates from the same women that were found only in the rectum. Twelve genetic regions occurred more often in multisite isolates than in rectal site-only isolates. Eleven of these 12 genetic regions are homologous to regions in the sequenced uropathogenic E. coli CFT073 strain.

* Corresponding author. Mailing address: Department of Epidemiology, 109 Observatory Street, Ann Arbor, MI 48109-2029. Phone: (734) 647-2407. Fax: (734) 764-3192. E-mail: cfmarrs{at}umich.edu.

Journal of Clinical Microbiology, July 2006, p. 2434-2441, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.00397-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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