JCM Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gopaul, K. K.
Right arrow Articles by Drobniewski, F. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gopaul, K. K.
Right arrow Articles by Drobniewski, F. A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 2006, p. 2492-2498, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.01428-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Progression Toward an Improved DNA Amplification-Based Typing Technique in the Study of Mycobacterium tuberculosis Epidemiology

Krishna K. Gopaul, Timothy J. Brown, Andrea L. Gibson, Malcolm D. Yates, and Francis A. Drobniewski*

Health Protection Agency Mycobacterium Reference Unit, Clinical Research Centre, Barts and the London Medical School, Queen Mary College, University of London, 2 Newark Street, London E1 2AT, United Kingdom

Received 14 July 2005/ Returned for modification 13 February 2006/ Accepted 14 May 2006

While high-copy-number IS6110-based restriction fragment length polymorphism (HCN-RFLP) is the gold standard for typing most Mycobacterium tuberculosis strains, the time taken for culturing and low throughput make it impractical for large-scale prospective typing of large numbers of isolates. The development of a new method, mycobacterial interspersed repetitive units (MIRU), a variation of the original variable-number tandem repeat (VNTR) technique, may provide a viable alternative. Panels based on the original 12-loci MIRU (12MIRU), a combination of 12MIRU and remaining ETR loci (15MIRU-VNTR), and an extended panel with an additional 10 novel regions (25VNTR) were used to study three populations with varying degrees of epidemiological data. MIRU discrimination increased with panel size and the addition of spoligotyping. Combining these two techniques enabled a reduction in the panel size from 25 to 14 loci without a significant loss in discrimination. However, 25VNTR alone or in combination with spoligotyping still possessed weaker discrimination than RFLP for high-copy-number isolates.

* Corresponding author. Mailing address: Health Protection Agency Mycobacterium Reference Unit, Clinical Research Centre, Barts and the London Medical School, Queen Mary College, University of London, 2 Newark St., London E1 2AT, United Kingdom. Phone: 44 (0) 20 7377 4895. Fax: 44 (0) 20 7539 3459. E-mail: f.drobniewski{at}qmul.ac.uk.

Journal of Clinical Microbiology, July 2006, p. 2492-2498, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.01428-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2006 by the American Society for Microbiology. All rights reserved.