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Journal of Clinical Microbiology, July 2006, p. 2567-2570, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.00495-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Culture-Independent Molecular Subtyping of Mycoplasma pneumoniae in Clinical Samples

Roger Dumke,1* Paul Christian Lück,1 Christoph Noppen,2 Christoph Schaefer,2 Heike von Baum,3 Reinhard Marre,3 and Enno Jacobs1

Technical University Dresden, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Fetscherstrasse 74, D-01307 Dresden, Germany,1 Viollier AG, Spalenring 145/147, CH-4002 Basel, Switzerland,2 Universitätsklinikum Ulm, Department of Medical Microbiology and Hygiene, Albert-Einstein-Allee 11, D-89081 Ulm, Germany3

Received 7 March 2006/ Returned for modification 28 March 2006/ Accepted 9 April 2006

A new molecular subtyping approach was developed which is based on the amplification and sequencing of a repetitive region of the P1 gene of Mycoplasma pneumoniae. It allows the differentiation of all known subtypes and variants of M. pneumoniae as well as the identification of new subtypes directly in clinical samples to characterize endemic and epidemic M. pneumoniae infections.

* Corresponding author. Mailing address: Technical University Dresden, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Fetscherstrasse 74, D-01307 Dresden, Germany. Phone: 49 351 4586577. Fax: 49 351 4586310. E-mail: roger.dumke{at}mailbox.tu-dresden.de.

Journal of Clinical Microbiology, July 2006, p. 2567-2570, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.00495-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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