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Journal of Clinical Microbiology, July 2006, p. 2567-2570, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.00495-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Culture-Independent Molecular Subtyping of Mycoplasma pneumoniae in Clinical Samples

Technical University Dresden, Medical Faculty Carl Gustav Carus, Institute of Medical Microbiology and Hygiene, Fetscherstrasse 74, D-01307 Dresden, Germany,¹ Viollier AG, Spalenring 145/147, CH-4002 Basel, Switzerland,² Universitätsklinikum Ulm, Department of Medical Microbiology and Hygiene, Albert-Einstein-Allee 11, D-89081 Ulm, Germany³

Received 7 March 2006/ Returned for modification 28 March 2006/ Accepted 9 April 2006

A new molecular subtyping approach was developed which is based on the amplification and sequencing of a repetitive region of the P1 gene of Mycoplasma pneumoniae. It allows the differentiation of all known subtypes and variants of M. pneumoniae as well as the identification of new subtypes directly in clinical samples to characterize endemic and epidemic M. pneumoniae infections.

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