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Journal of Clinical Microbiology, July 2006, p. 2590-2592, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.00125-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Amplification of the Hyphal Wall Protein 1 Gene To Distinguish Candida albicans from Candida dubliniensis

Orazio Romeo, Cosimo Racco, and Giuseppe Criseo*

Department of Microbiological, Genetic, and Molecular Sciences, University of Messina, Salita Sperone, 31 98166 Messina, Italy

Received 20 January 2006/ Returned for modification 2 March 2006/ Accepted 9 May 2006

The authors developed a new, simple, and reliable PCR/restriction fragment length polymorphism technique, using amplification of the hyphal wall protein 1 gene of Candida albicans and its gene homologue in Candida dubliniensis, to differentiate the two species of Candida. Performed with a new primer set, CRR-f/CRR-r, PCR produced two different fragments: one of 1,180 bp for C. albicans, and one of 930 bp for C. dubliniensis.

* Corresponding author. Mailing address: Dipartimento di Scienze Microbiologiche, Genetiche e Molecolari, Università degli studi di Messina, Salita Sperone, 31 98166 Messina, Italy. Phone: 39-0906765195. Fax: 39-090392733. E-mail: gcriseo{at}unime.it.

Journal of Clinical Microbiology, July 2006, p. 2590-2592, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.00125-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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