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Journal of Clinical Microbiology, July 2006, p. 2605-2608, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.00752-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Rapid Genotypic Detection of Rifampin- and Isoniazid-Resistant Mycobacterium tuberculosis Directly in Clinical Specimens

Didi Bang,1* Åse Bengård Andersen,2 and Vibeke Østergaard Thomsen1

International Reference Laboratory of Mycobacteriology, Statens Serum Institut, National Institute for Prevention and Control of Infectious Diseases and Congenital Disorders, Artillerivej 5, 2300 Copenhagen, Denmark,1 Department of Infectious Diseases, Rigshospitalet, Blegdamsvej 9, 2100 Copenhagen, Denmark2

Received 9 April 2006/ Accepted 14 May 2006

A multiplex PCR DNA strip assay (Genotype MTBDR) designed to detect rifampin (rpoB) and high-level isoniazid (katG) resistance mutations in Mycobacterium tuberculosis isolates was optimized for clinical specimens. Successful genotypic results were achieved with 36 of 38 (95%) smear-positive respiratory specimens, allowing rapid therapeutic adjustments in transmittable drug-resistant tuberculosis.

* Corresponding author. Mailing address: Didi Bang, International Reference Laboratory of Mycobacteriology, Statens Serum Institut, Artillerivej 5, 2300 Copenhagen S, Denmark. Phone: 45 3268 3705. Fax: 45 3268 3871. E-mail: DVB{at}ssi.dk.

Journal of Clinical Microbiology, July 2006, p. 2605-2608, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.00752-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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