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Journal of Clinical Microbiology, July 2006, p. 2609-2611, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.00572-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Identification of Bacteria in Formalin-Fixed, Paraffin-Embedded Heart Valve Tissue via 16S rRNA Gene Nucleotide Sequencing

Kavita Imrit,2,3 Michael Goldfischer,1 Jie Wang,1 Jaime Green,3 Jerome Levine,2 Joseph Lombardo,1 and Tao Hong1*

Department of Pathology,1 Division of Infectious Diseases, Hackensack University Medical Center, Hackensack, New Jersey,2 University of Medicine and Dentistry of New Jersey, Newark, New Jersey3

Received 16 March 2006/ Returned for modification 12 April 2006/ Accepted 18 April 2006

We applied 16S rRNA gene sequencing to identify bacterial species present in formalin-fixed, paraffin-embedded heart valve tissue. In 40% (12/30) of the cases, we were able to identify the bacterium to the species-genus level. For more recent cases (≤4 years), the success rate was significantly improved, to 70% (P < 0.001).

* Corresponding author. Mailing address: Department of Pathology, Clinical Microbiology Laboratory, Hackensack University Medical Center, 30 Prospect Ave., Hackensack, NJ 07601. Phone: (201) 996-4854. Fax: (201) 996-2156. E-mail: thong{at}humed.com.

Journal of Clinical Microbiology, July 2006, p. 2609-2611, Vol. 44, No. 7
0095-1137/06/$08.00+0     doi:10.1128/JCM.00572-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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