This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Layer, F.
Right arrow Articles by König, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Layer, F.
Right arrow Articles by König, B.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 2006, p. 2824-2830, Vol. 44, No. 8
0095-1137/06/$08.00+0     doi:10.1128/JCM.00226-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Comparative Study Using Various Methods for Identification of Staphylococcus Species in Clinical Specimens

F. Layer, B. Ghebremedhin, K.-A. Moder, W. König, and B. König*

Otto von Guericke University, Institute of Medical Microbiology, Leipziger Str. 44, D-39120 Magdeburg, Germany

Received 1 February 2006/ Returned for modification 13 March 2006/ Accepted 1 June 2006

Coagulase-negative staphylococci (CNS) play a predominant role in nosocomial infections. Rapid, reliable identification of these organisms is essential for accurate diagnosis and prompt effective treatment of these infections. Quite recently, the VITEK 2 g-positive (gram-positive [GP]) identification card (bioMérieux) has been redesigned for greater accuracy in the identification of gram-positive cocci. We compared the BD Phoenix (Becton Dickinson) and VITEK 2 (bioMérieux) automated microbiology systems, using their respective update version cards, and the API ID32 STAPH test. The glyceraldehyde-3-phosphate dehydrogenase (gap) gene-based T-RFLP (terminal restriction fragment length polymorphism) method was used for verifying the results. In total, 86 clinical isolates of CNS and 27 reference strains were analyzed. The results show that for identification of CNS, the automated identification methods using the newest VITEK 2 and BD Phoenix identification cards are comparable. However, API ID32 STAPH revealed more correct results compared to both automated microbiology systems. Despite the increased performance of the phenotypic automated identification systems compared to the former versions, molecular methods, e.g., the gap-based T-RFLP method, still show superior accuracy in identifying Staphylococcus species other than Staphylococcus aureus.

* Corresponding author. Mailing address: Otto-von Guericke University, Institute of Medical Microbiology, Leipziger Str. 44, D-39120 Magdeburg, Germany. Phone: 493916713353. Fax: 493916713538. E-mail: Brigitte.koenig{at}medizin.uni-magdeburg.de.

Journal of Clinical Microbiology, August 2006, p. 2824-2830, Vol. 44, No. 8
0095-1137/06/$08.00+0     doi:10.1128/JCM.00226-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Brigante, G., Menozzi, M. G., Pini, B., Porta, R., Somenzi, P., Sciacca, A., Spanu, T., Stefani, S. (2008). Identification of Coagulase-Negative Staphylococci by Using the BD Phoenix System in the Low-Inoculum Mode. J. Clin. Microbiol. 46: 3826-3828 [Abstract] [Full Text]  
  • Yu, D., Chen, Y., Pan, Y., Li, H., McCormac, M. A., Tang, Y.-W. (2008). Staphylococcus gallinarum Bacteremia in a Patient with Chronic Hepatitis B Virus Infection. Annals of Clinical & Laboratory Science 38: 401-404 [Abstract] [Full Text]  
  • Delmas, J., Chacornac, J. P., Robin, F., Giammarinaro, P., Talon, R., Bonnet, R. (2008). Evaluation of the Vitek 2 System with a Variety of Staphylococcus Species. J. Clin. Microbiol. 46: 311-313 [Abstract] [Full Text]  
  • Chiquet, C., Pechinot, A., Creuzot-Garcher, C., Benito, Y., Croize, J., Boisset, S., Romanet, J. P., Lina, G., Vandenesch, F., for the French Institutional Endophthalmitis Study, (2007). Acute Postoperative Endophthalmitis Caused by Staphylococcus lugdunensis. J. Clin. Microbiol. 45: 1673-1678 [Abstract] [Full Text]  
  • Tang, Y.-W., Kilic, A., Yang, Q., McAllister, S. K., Li, H., Miller, R. S., McCormac, M., Tracy, K. D., Stratton, C. W., Han, J., Limbago, B. (2007). StaphPlex System for Rapid and Simultaneous Identification of Antibiotic Resistance Determinants and Panton-Valentine Leukocidin Detection of Staphylococci from Positive Blood Cultures. J. Clin. Microbiol. 45: 1867-1873 [Abstract] [Full Text]  
  • Sasaki, T., Kikuchi, K., Tanaka, Y., Takahashi, N., Kamata, S., Hiramatsu, K. (2007). Methicillin-Resistant Staphylococcus pseudintermedius in a Veterinary Teaching Hospital. J. Clin. Microbiol. 45: 1118-1125 [Abstract] [Full Text]  
  • Gilad, J., Schwartz, D., Layer, F., Ghebremedhin, B., Moder, K. A., Konig, W., Konig, B. (2007). Identification of Staphylococcus Species with the VITEK 2 System: the Case of Staphylococcus hominis. J. Clin. Microbiol. 45: 685-686 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»