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Journal of Clinical Microbiology, August 2006, p. 2831-2837, Vol. 44, No. 8
0095-1137/06/$08.00+0     doi:10.1128/JCM.00418-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Characterization of a Large Outbreak by CTX-M-1-Producing Klebsiella pneumoniae and Mechanisms Leading to In Vivo Carbapenem Resistance Development

Ana Mena,1 Virginia Plasencia,1 Laura García,2 Olga Hidalgo,3 José Ignacio Ayestarán,4 Sebastián Alberti,2 Nuria Borrell,1 José L. Pérez,1 and Antonio Oliver1*

Servicio de Microbiología, Hospital Son Dureta and Instituto Universitario de Investigación en Ciencias de la Salud,1 Área de Microbiología and Instituto Universitario de Investigación en Ciencias de la Salud, Universidad de las Islas Baleares,2 Servicio de Medicina Preventiva, Hospital Son Dureta,3 Servicio de Medicina Intensiva, Hospital Son Dureta, Palma de Mallorca, Spain4

Received 26 February 2006/ Returned for modification 25 April 2006/ Accepted 2 June 2006

All extended-spectrum ß-lactamase (ESBL)-producing Enterobacteriaceae isolates from patients admitted to and adult intensive care unit were prospectively documented from 2002 to 2005, when a large outbreak (51 patients affected) of multiresistant ESBL-producing Klebsiella pneumoniae infection was detected. The involvement of a single K. pneumoniae clone was demonstrated by pulsed-field gel electrophoresis. In addition to the ESBL-mediated resistance, the epidemic strain uniformly showed cross-resistance to ciprofloxacin, gentamicin, tobramycin, trimethoprim-sulfamethoxazole, and tetracycline, whereas resistance to the ß-lactam-ß-lactamase inhibitor combinations was variable. The ESBL involved was CTX-M-1, as demonstrated by isoelectric focusing, PCR amplification, and sequencing. CTX-M-1 as well as the aminoglycoside resistance determinants were encoded in a 50-kb plasmid that could be transferred to Escherichia coli only by transformation. In two of the infected patients, carbapenem resistance development (MICs of 8 to 12, 16, and >32 µg/ml for imipenem, meropenem, and ertapenem, respectively) was documented, both in clinical samples and in intestinal colonization studies. The analysis of the outer membrane proteins of the carbapenem-susceptible and -resistant isolates revealed that the former expressed only one of the two major porins, OmpK36, whereas the latter did not express either of them. In one of the cases, the lack of expression of OmpK36 was demonstrated to be mediated by the interruption of the coding sequence by the insertion sequence IS26. This is the first report of a large outbreak of CTX-M-1-producing Enterobacteriaceae and, curiously, the first documented description in the literature of CTX-M-1 in K. pneumoniae, despite the fact that this enzyme has been found in multiple species. Furthermore, we document and characterize for the first time carbapenem resistance development in CTX-M-1-producing Enterobacteriaceae.

* Corresponding author. Mailing address: Servicio de Microbiología, Hospital Son Dureta, C. Andrea Doria no. 55, 07014 Palma de Mallorca, Spain. Phone: 34 971 175 185. Fax: 34 971 175 185. E-mail: aoliver{at}hsd.es.

Journal of Clinical Microbiology, August 2006, p. 2831-2837, Vol. 44, No. 8
0095-1137/06/$08.00+0     doi:10.1128/JCM.00418-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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