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Journal of Clinical Microbiology, August 2006, p. 2970-2973, Vol. 44, No. 8
0095-1137/06/$08.00+0     doi:10.1128/JCM.00584-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Short-Sequence-Repeat Analysis of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium Isolates Collected from Animals Throughout the United States Reveals Both Stability of Loci and Extensive Diversity

N. Beth Harris,1 Janet B. Payeur,1 Vivek Kapur,2 and Srinand Sreevatsan3*

National Veterinary Services Laboratories, Ames, IA 50010,1 Department of Microbiology,2 Center for Genomics and Veterinary Population Medicine Department, University of Minnesota, St. Paul, Minnesota 551083

Received 20 March 2006/ Returned for modification 25 April 2006/ Accepted 18 May 2006

We analyzed the multilocus short sequence repeats (SSRs) of 211 and 56 isolates of Mycobacterium avium subsp. paratuberculosis and M. avium subsp. avium, respectively. The M. avium subsp. paratuberculosis isolates could be differentiated into 61 genotypes. The M. avium subsp. avium isolates showed limited diversity. These SSRs are stable and suitable for studying the molecular epidemiology of M. avium subsp. paratuberculosis.

* Corresponding author. Mailing address: Veterinary Population Medicine Department, University of Minnesota, 136G Andrew Boss Laboratory for Meat Hygiene, 1354 Eckles Avenue, St. Paul, MN 55108. Phone: (612) 625-3769. Fax: (612) 624-4906. E-mail: sreev001{at}umn.edu.

Journal of Clinical Microbiology, August 2006, p. 2970-2973, Vol. 44, No. 8
0095-1137/06/$08.00+0     doi:10.1128/JCM.00584-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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Copyright © 2006 by the American Society for Microbiology. All rights reserved.