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Journal of Clinical Microbiology, August 2006, p. 3008-3011, Vol. 44, No. 8
0095-1137/06/$08.00+0     doi:10.1128/JCM.02576-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Pyrosequencing as a Rapid Tool for Identification of GES-Type Extended-Spectrum ß-Lactamases

Laurent Poirel, Thierry Naas, and Patrice Nordmann*

Service de Bactériologie-Virologie, Hôpital de Bicêtre, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine Paris-Sud, Université Paris XI, 94275 K.-Bicêtre, France

Received 12 December 2005/ Returned for modification 15 May 2006/ Accepted 5 June 2006

A pyrosequencing technique was used for identification of extended-spectrum ß-lactamases (ESBLs) of GES type. These ß-lactamases are isolated increasingly emerging in gram-negative bacteria worldwide. This rapid and reliable identification method is interesting, since GES variants, including not only expanded-spectrum cephalosporins but also carbapenems, cephamycins, and monobactams, are the only ESBLs that possess different hydrolysis profiles.

* Corresponding author. Mailing address: Service de Bactériologie-Virologie, Hôpital de Bicêtre, 78 rue du Général Leclerc, 94275 Le Kremlin-Bicêtre cedex, France. Phone: 33-1-45-21-36-32. Fax: 33-1-45-21-63-40. E-mail: nordmann.patrice{at}bct.aphp.fr.

Journal of Clinical Microbiology, August 2006, p. 3008-3011, Vol. 44, No. 8
0095-1137/06/$08.00+0     doi:10.1128/JCM.02576-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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