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Journal of Clinical Microbiology, August 2006, p. 3028-3030, Vol. 44, No. 8
0095-1137/06/$08.00+0     doi:10.1128/JCM.00913-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Clinical Evaluation of a Type III Secretion System Real-Time PCR Assay for Diagnosing Melioidosis

Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia,¹ Bacterial Zoonoses Branch, Division of Foodborne, Bacterial, and Mycotic Diseases, National Center for Zoonotic, Vectorborne, and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333,² Infectious Diseases Unit, Royal Darwin Hospital and Northern Territory Clinical School, Darwin, Northern Territory, Australia³

Received 2 May 2006/ Returned for modification 25 May 2006/ Accepted 15 June 2006

A Burkholderia pseudomallei type III secretion system real-time PCR assay was evaluated on clinical specimens in a region where melioidosis is endemic. The PCR was positive in 30/33 (91%) patients with culture-confirmed melioidosis. All six patients with melioidosis septic shock were blood PCR positive, suggesting potential for rapid diagnosis and commencement of appropriate therapy.

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