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Journal of Clinical Microbiology, September 2006, p. 3055-3064, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00187-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Genetic Variability in the G Protein Gene of Group A and B Respiratory Syncytial Viruses from India

Shama Parveen,1 Wayne M. Sullender,3 Karen Fowler,3,4,5 Elliot J. Lefkowitz,6 Suresh Kumar Kapoor,2 and Shobha Broor1*

Department of Microbiology,1 Comprehensive Rural Health Services Project, Centre for Community Medicine, All India Institute of Medical Sciences, New Delhi, India, and Departments of,2 Pediatrics,3 Epidemiology,4 Maternal and Child Health,5 Microbiology, University of Alabama at Birmingham, Birmingham, Alabama6

Received 28 January 2006/ Returned for modification 17 March 2006/ Accepted 6 May 2006

Respiratory syncytial virus (RSV) is the most commonly identified viral agent of acute respiratory tract infection (ARI) of young children and causes repeat infections throughout life. Limited data are available on the molecular epidemiology of RSV from developing countries, including India. This study reports on the genetic variability in the glycoprotein G gene among RSV isolates from India. Reverse transcription-PCR for a region of the RSV G protein gene was done with nasopharyngeal aspirates (NPAs) collected in a prospective longitudinal study in two rural villages near Delhi and from children with ARI seen in an urban hospital. Nucleotide sequence comparisons among 48 samples demonstrated a higher prevalence of group A (77%) than group B (23%) RSV isolates. The level of genetic variability was higher among the group A viruses (up to 14%) than among the group B viruses (up to 2%). Phylogenetic analysis revealed that both the GA2 and GA5 group A RSV genotypes were prevalent during the 2002-2003 season and that genotype GA5 was predominant in the 2003-2004 season, whereas during the 2004-2005 season both genotype GA5 and genotype BA, a newly identified group B genotype, cocirculated in almost equal proportions. Comparison of the nonsynonymous mutation-to-synonymous mutation ratios (dN/dS) revealed greater evidence for selective pressure between the GA2 and GA5 genotypes (dN/dS, 1.78) than within the genotypes (dN/dS, 0.69). These are among the first molecular analyses of RSV strains from the second most populous country in the world and will be useful for comparisons to candidate vaccine strains.

* Corresponding author. Mailing address: Department of Microbiology, All India Institute of Medical Sciences, New Delhi 110029, India. Phone: 91 11 2659 4926. Fax: 91 11 2658 8663. E-mail: shobha.broor{at}gmail.com.

Journal of Clinical Microbiology, September 2006, p. 3055-3064, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00187-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Reiche, J., Schweiger, B. (2009). Genetic Variability of Group A Human Respiratory Syncytial Virus Strains Circulating in Germany from 1998 to 2007. J. Clin. Microbiol. 47: 1800-1810 [Abstract] [Full Text]  
  • Zlateva, K. T., Vijgen, L., Dekeersmaeker, N., Naranjo, C., Van Ranst, M. (2007). Subgroup Prevalence and Genotype Circulation Patterns of Human Respiratory Syncytial Virus in Belgium during Ten Successive Epidemic Seasons. J. Clin. Microbiol. 45: 3022-3030 [Abstract] [Full Text]  


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