This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Park, S.-J.
Right arrow Articles by Cho, K.-O.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Park, S.-J.
Right arrow Articles by Cho, K.-O.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, September 2006, p. 3178-3188, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.02667-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Detection and Characterization of Bovine Coronaviruses in Fecal Specimens of Adult Cattle with Diarrhea during the Warmer Seasons

Su-Jin Park,1 Cheol Jeong,1 Soon-Seek Yoon,2 Hyoun E. Choy,3 Linda J. Saif,4 Sung-Hee Park,1 You-Jung Kim,1 Jae-Ho Jeong,1 Sang-Ik Park,1 Ha-Hyun Kim,1 Bong-Joo Lee,1 Ho-Seong Cho,1 Sang-Ki Kim,1 Mun-Il Kang,1 and Kyoung-Oh Cho1*

Biotherapy Human Resources Center, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757,1 Pathology Division, National Veterinary Research and Quarantine Service, Anyang, Kyunggi 430-824,2 Genome Research Center for Enteropathogenic Bacteria, Chonnam National University Medical College, Gwangju 501-746, South Korea,3 Food Animal Health Research Program, Department of Veterinary Preventive Medicine, Ohio Agricultural Research and Development Center, The Ohio State University, Wooster, Ohio 446914

Received 23 December 2005/ Returned for modification 15 February 2006/ Accepted 11 June 2006

Bovine coronavirus (BCoV) is an etiological agent associated with winter dysentery (WD), prevalent in adult cattle during the winter. Although we previously detected, isolated, and characterized BCoV strains from adult cattle with WD (WD-BCoV strains) during the winter in South Korea, the precise epidemiology, as well as the causative agent of diarrhea in adult cattle in the warmer seasons, has not been examined. We examined 184 diarrheic fecal specimens collected from 75 herds of adult cattle from seven provinces during the spring (warm), autumn (warm), and summer (hot) seasons. Bovine coronavirus-positive reactions were detected for 107 (58.2%) diarrheic fecal samples (in 47/75 herds). Of these 107 positive samples, 90 fecal samples from 33 herds tested positive for BCoV alone and 17 fecal samples from 14 herds also tested positive for other pathogens. Biological comparisons between the 9 BCoV strains isolated in this study and the 10 previously isolated WD-BCoV strains revealed that there was no receptor-destroying enzyme (RDE) activity against mouse erythrocytes in the 9 BCoV strains but the 10 WD-BCoV strains had high RDE activity. Phylogenetic analysis of the spike (S) and hemagglutinin/esterase (HE) proteins revealed that all the Korean BCoVs clustered together regardless of season and were distinct from the other known BCoVs, suggesting a distinct evolutionary pathway for the Korean BCoVs. These and previous results revealed a high prevalence and widespread geographical distribution of BCoV, suggesting that this virus is endemic in adult cattle with diarrhea in all seasons in South Korea.

* Corresponding author. Mailing address: Biotherapy Human Resources Center, College of Veterinary Medicine, Chonnam National University, Gwangju 500-757, South Korea. Phone: 82-62-530-2845. Fax: 82-62-530-0835. E-mail: choko{at}chonnam.ac.kr.

Journal of Clinical Microbiology, September 2006, p. 3178-3188, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.02667-05
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Alekseev, K. P., Vlasova, A. N., Jung, K., Hasoksuz, M., Zhang, X., Halpin, R., Wang, S., Ghedin, E., Spiro, D., Saif, L. J. (2008). Bovine-Like Coronaviruses Isolated from Four Species of Captive Wild Ruminants Are Homologous to Bovine Coronaviruses, Based on Complete Genomic Sequences. J. Virol. 82: 12422-12431 [Abstract] [Full Text]  
  • Genova, S. G., Streeter, R. N., Simpson, K. M., Kapil, S. (2008). Detection of an Antigenic Group 2 Coronavirus in an Adult Alpaca with Enteritis. CVI 15: 1629-1632 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»