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Journal of Clinical Microbiology, September 2006, p. 3201-3207, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00656-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

An Outbreak of Keratitis Caused by Mycobacterium immunogenum

Jorge Luiz Mello Sampaio,1,3* Doraldo Nassar Junior,2 Denise de Freitas,2 Ana Luisa Höfling-Lima,2 Kozue Miyashiro,3 Fernando Lopes Alberto,3 and Sylvia Cardoso Leão1

Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, Brazil,1 Departamento de Oftalmologia, Universidade Federal de São Paulo, São Paulo, Brazil,2 Instituto Fleury de Ensino e Pesquisa, São Paulo, Brazil3

Received 27 March 2006/ Returned for modification 7 June 2006/ Accepted 11 July 2006

From 8 October to 12 November 2003, 36 patients underwent surgical correction of myopia in a São Paulo, Brazil, clinic. Five patients had clinical signs of infectious keratitis, and a Mycobacterium species with previously unreported patterns determined by PCR restriction enzyme analysis of the hsp65 gene and PCR restriction enzyme analysis of the 16S-23S rRNA internal transcribed spacer (ITS) was isolated from corneal scrapings from four of these patients. Subsequent evaluation by phenotypic tests and partial sequencing of the hsp65, sodA, rpoB, and 16S rRNA genes and the ITS supported the species identification as a variant of Mycobacterium immunogenum. The source of infection was not determined. The outbreak was caused by a single clone, as evidenced by identical pulsed-field gel electrophoresis and enterobacterial repetitive intergenic consensus-PCR profiles. This is the first report of an outbreak where this species was isolated from infected tissues.

* Corresponding author. Mailing address: Universidade Federal de São Paulo—Escola Paulista de Medicina, Departamento de Microbiologia, Imunologia e Parasitologia, Rua Botucatu, 862—3° andar, 04023-062 São Paulo, Brazil. Phone: 55 11 50147730. Fax: 55 11 50147601. E-mail: sampaio{at}ecb.epm.br.

Journal of Clinical Microbiology, September 2006, p. 3201-3207, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00656-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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