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Journal of Clinical Microbiology, September 2006, p. 3340-3345, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00213-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Evaluation of the New Chromogenic Medium Candida ID 2 for Isolation and Identification of Candida albicans and Other Medically Important Candida Species

Elena Eraso,1 María D. Moragues,2 María Villar-Vidal,1 Ismail H. Sahand,1 Nagore González-Gómez,1 José Pontón,1 and Guillermo Quindós1*

Laboratorio de Micología Médica, Departamento de Inmunología, Microbiología y Parasitología, Facultad de Medicina y Odontología,1 Departamento de Enfermería I, Universidad del País Vasco-Euskal Herriko Unibertsitatea, Apartado 699, 48080 Bilbao, Spain2

Received 31 January 2006/ Returned for modification 13 March 2006/ Accepted 1 June 2006

The usefulness of Candida ID 2 (CAID2) reformulated medium (bioMérieux, France) has been compared with that of the former Candida ID (CAID; bioMérieux), Albicans ID 2 (ALB2; bioMérieux), and CHROMagar Candida (CAC; Chromagar, France) chromogenic media for the isolation and presumptive identification of clinically relevant yeasts. Three hundred forty-five stock strains from culture collections, and 103 fresh isolates from different clinical specimens were evaluated. CAID2 permitted differentiation based on colony color between Candida albicans (cobalt blue; sensitivity, 91.7%; specificity, 97.2%) and Candida dubliniensis (turquoise blue; sensitivity, 97.9%; specificity, 96.6%). Candida tropicalis gave distinguishable pink-bluish colonies in 97.4% of the strains in CAID2 (sensitivity, 97.4%; specificity, 100%); the same proportion was reached in CAC, where colonies were blue-gray (sensitivity, 97.4%; specificity, 98.7%). CAC and CAID2 showed 100% sensitivity values for the identification of Candida krusei. However, with CAID2, experience is required to differentiate the downy aspect of the white colonies of C. krusei from other white-colony-forming species. The new CAID2 medium is a good candidate to replace CAID and ALB2, and it compares well to CAC for culture and presumptive identification of clinically relevant Candida species. CAID2 showed better results than CAC in some aspects, such as quicker growth and color development of colonies from clinical specimens, detection of mixed cultures, and presumptive differentiation between C. albicans and C. dubliniensis.

* Corresponding author. Mailing address: Laboratorio de Micología Médica, Departamento de Inmunología, Microbiología, y Parasitología, Facultad de Medicina y Odontología, Universidad del País Vasco-Euskal Herriko Unibertsitatea, Apartado 699, E-48080 Bilbao, Spain. Phone: 34 94 6012854. Fax: 34 94 6013400. E-mail: guillermo.quindos{at}ehu.es.

Journal of Clinical Microbiology, September 2006, p. 3340-3345, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00213-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Sahand, I. H., Maza, J. L., Eraso, E., Montejo, M., Moragues, M. D., Aguirre, J. M., Quindos, G., Ponton, J. (2009). Evaluation of CHROM-Pal medium for the isolation and direct identification of Candida dubliniensis in primary cultures from the oral cavity. J Med Microbiol 58: 1437-1442 [Abstract] [Full Text]  


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