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Journal of Clinical Microbiology, September 2006, p. 3371-3373, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.01289-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Molecular Epidemiology of Adenovirus Isolates from Patients Diagnosed with Intussusception in Melbourne, Australia

Gowri Selvaraj,1 Carl Kirkwood,1,3 Julie Bines,1,2,3 and Jim Buttery1,2,3*

Enteric Virus Group,1 Centre of Clinical Research Excellence in Child and Adolescent Immunisation, Murdoch Children's Research Institute,2 Department of Paediatrics, University of Melbourne, Royal Children's Hospital, Melbourne, Australia3

Received 22 June 2006/ Accepted 10 July 2006

Twenty-one intussusception (IS)-associated and 59 temporally linked adenoviral isolates from clinical infections were compared. Species C (15/21 IS- and 32/59 non-IS-associated isolates) dominated. Of these, serotype 2 (AdV-2) (7/15 IS-associated isolates) and serotype 1 (AdV-1) (16/32 non-IS-associated isolates) were the most commonly identified serotypes. DNA restriction analysis of AdV-2 isolates identified six genomic types; of these, type D2 (3/7 IS- and 8/11 non-IS-associated isolates) was the dominant type after BamHI and SmaI digestion. IS-associated isolates are similar to circulating non-IS-associated strains.

* Corresponding author. Mailing address: Immunisation CCRE, Murdoch Children's Research Institute, Royal Children's Hospital, Level 4, FEB, Flemington Rd., Parkville, Melbourne, Victoria 3052, Australia. Phone: 61 (0)3 9345 4772. Fax: 61 (0)3 9345 4751. E-mail: jim.buttery{at}rch.org.au.

Journal of Clinical Microbiology, September 2006, p. 3371-3373, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.01289-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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