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Journal of Clinical Microbiology, September 2006, p. 3388-3390, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00972-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Comparison of API 20E and invA PCR for Identification of Salmonella enterica Isolates from Swine Production Units

Daniele M. Nucera, Carol W. Maddox, Patricia Hoien-Dalen, and Ronald M. Weigel*

Department of Pathobiology, University of Illinois, Urbana, Illinois

Received 9 May 2006/ Accepted 4 July 2006

API 20E and invA PCR were evaluated for the identification of Salmonella enterica isolates from swine farms. API 20E had the highest agreement with other tests at the 99.9% likelihood level. Both tests had 100% sensitivity and 96% specificity compared to 16S rRNA sequencing. Compared to serotyping, both tests had 96% sensitivity; specificity was 86% for API 20E and 79% for invA PCR.

* Corresponding author. Mailing address: Department of Pathobiology, University of Illinois, 2001 South Lincoln Avenue, Urbana, IL 61802. Phone: (217) 244-1365. Fax: (217) 244-7441. E-mail: weigel{at}uiuc.edu.

Journal of Clinical Microbiology, September 2006, p. 3388-3390, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00972-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.



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