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Journal of Clinical Microbiology, September 2006, p. 3405-3408, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00730-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Molecular Epidemiology of Multidrug-Resistant Mycobacterium bovis Isolates with the Same Spoligotyping Profile as Isolates from Animals

Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, 28040 Madrid, Spain,¹ Servicio de Microbiología, Hospital Ramón y Cajal, IMSALUD, 28034 Madrid, Spain²

Received 6 April 2006/ Returned for modification 8 May 2006/ Accepted 6 July 2006

PCR-based characterization techniques have been adopted in most laboratories for Mycobacterium bovis typing. We report a molecular characterization of human multidrug-resistant M. bovis isolates and three bovine isolates that share the spoligotyping profile. The analysis of the direct repeat region showed that both groups differed in the presence of spacers not included in the current membrane. They were also distinguished by two out of the nine mycobacterial interspersed repetitive unit variable-number tandem repeat loci tested, indicating that the human infection was not acquired from the cattle from which isolates were obtained. These results highlight that a combination of techniques is required for appropriate discrimination, even for those spoligotypes that have a low frequency.

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• Romero, B., Aranaz, A., Bezos, J., Alvarez, J., de Juan, L., Mateos, A., Gomez-Mampaso, E., Dominguez, L. (2008). Single-Nucleotide Polymorphism in Two Representative Multidrug-Resistant Mycobacterium bovis Isolates Collected from Patients in a Spanish Hospital Harboring a Human Infection Outbreak. J. Clin. Microbiol. 46: 826-827 [Full Text]