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Journal of Clinical Microbiology, September 2006, p. 3415-3417, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.00508-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Fluorescence In Situ Hybridization for Rapid Identification of Achromobacter xylosoxidans and Alcaligenes faecalis Recovered from Cystic Fibrosis Patients

Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Ulm, Germany

Received 8 March 2006/ Returned for modification 2 June 2006/ Accepted 4 July 2006

Achromobacter xylosoxidans is frequently isolated from the respiratory secretions of cystic fibrosis (CF) patients, but identification with biochemical tests is unreliable. We describe fluorescence in situ hybridization assays for the rapid identification of Achromobacter xylosoxidans and Alcaligenes faecalis. Both assays showed high sensitivities and high specificities with a collection of 155 nonfermenters from CF patients.

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