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Journal of Clinical Microbiology, September 2006, p. 3443-3447, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.01175-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.

Invader Plus Method Detects Herpes Simplex Virus in Cerebrospinal Fluid and Simultaneously Differentiates Types 1 and 2

Hatim T. Allawi,1* Haijing Li,2 Tamara Sander,1 Azamat Aslanukov,1 Victor I. Lyamichev,1 Amondrea Blackman,2 Slava Elagin,1 and Yi-Wei Tang2

Third Wave Technologies, Inc., Madison, Wisconsin 53719,1 Vanderbilt University Medical Center, Nashville, Tennessee 37232-53102

Received 7 June 2006/ Accepted 19 June 2006

We report here on the development and validation of a prototype Invader Plus method for the qualitative detection of herpes simplex virus types 1 and 2 in cerebrospinal fluid (CSF). The method combines PCR and Invader techniques in a single, closed-tube, continuous-reaction format that gives an analytical sensitivity of approximately 10 copies per reaction. The clinical sensitivity and specificity were 100.0% and 98.6%, respectively, when the results of the method were validated against the results obtained with a PCR colorimetric microtiter plate system by use of clinical CSF specimens.

* Corresponding author. Mailing address: Third Wave Technologies, Inc., 502 South Rosa Rd., Madison, WI 53719. Phone: (608) 273-8933, ext. 8203. Fax: (608) 273-8618. E-mail: hallawi{at}twt.com.

Journal of Clinical Microbiology, September 2006, p. 3443-3447, Vol. 44, No. 9
0095-1137/06/$08.00+0     doi:10.1128/JCM.01175-06
Copyright © 2006, American Society for Microbiology. All Rights Reserved.





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