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Journal of Clinical Microbiology, January 2007, p. 109-111, Vol. 45, No. 1
0095-1137/07/$08.00+0     doi:10.1128/JCM.01969-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Statistical Analyses of Correlation between Fluconazole MICs for Candida spp. Assessed by Standard Methods Set Forth by the European Committee on Antimicrobial Susceptibility Testing (E.Dis. 7.1) and CLSI (M27-A2){triangledown}

Juan Luis Rodriguez-Tudela,1* J. Peter Donnelly,2 Michael A. Pfaller,3 Erja Chryssantou,4 Peter Warn,5 David W. Denning,6 Ana Espinel-Ingroff,7 Francesco Barchiesi,8 and Manuel Cuenca-Estrella1

Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Majadahonda, Spain,1 Department of Haematology, Radboud University Nijmegen Medical Centre and Nijmegen University Centre for Infectious Diseases, Radboud University, Nijmegen, The Netherlands,2 Department of Pathology, University of Iowa College of Medicine, Iowa City, Iowa,3 Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, Sweden,4 School of Medicine, University of Manchester, Stopford Building, Oxford Road, Manchester, United Kingdom,5 Education and Research Centre, Wythenshawe Hospital, Southmoor Road, Manchester, United Kingdom,6 VCU Medical Center, 1101 E. Marshall St., Sanger Hall, Rm. 7-049, P.O. Box 980049, Richmond, Virginia,7 Istituto di Malattie Infettive e Medicina Pubblica, Università Politecnica delle Marche, Ancona, Italy8

Received 22 September 2006/ Returned for modification 17 October 2006/ Accepted 25 October 2006

The European Committee on Antimicrobial Susceptibility Testing (EUCAST) Subcommittee on Antifungal Susceptibility Testing recently published a standard for determining the susceptibility of fermentative yeasts to antifungals. From the beginning, the EUCAST and its North American counterpart, the CLSI, decided to work together in order to establish common standards. As part of this exercise, the susceptibility of a set of 475 yeast isolates was tested by both standards. The intraclass correlation coefficient and the equations defining the linear regression between both methods were estimated. Both methods produced very similar results, with an intraclass correlation coefficient of 0.954 (0.945 to 0.962), although linear regression analysis shows that the EUCAST standard resulted in slightly lower MICs. There were only eight isolates showing at least four twofold dilution MIC differences between both standards. After 24 h of incubation, the MICs obtained by the CLSI method were equivalent to those obtained by the EUCAST standard. In summary, both methods produce very similar MICs, indicating that methodology does not pose any obstacle to obtaining uniform standards for antifungal susceptibility testing of yeasts.

* Corresponding author. Mailing address: Servicio de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra. Majadahonda Pozuelo km 2, 28220 Majadahonda, Madrid, Spain. Phone: 34 91 822 3919. Fax: 34 91 509 7966. E-mail: juanl.rodriguez-tudela{at}isciii.es.

{triangledown} Published ahead of print on 8 November 2006.

Journal of Clinical Microbiology, January 2007, p. 109-111, Vol. 45, No. 1
0095-1137/07/$08.00+0     doi:10.1128/JCM.01969-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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