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Journal of Clinical Microbiology, January 2007, p. 222-223, Vol. 45, No. 1
0095-1137/07/$08.00+0     doi:10.1128/JCM.01732-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Quality Control Limits for Posaconazole Disk Susceptibility Tests on Mueller-Hinton Agar with Glucose and Methylene Blue{triangledown}

S. Brown* and M. Traczewski

The Clinical Microbiology Institute, Wilsonville, Oregon 97070

Received 21 August 2006/ Returned for modification 13 October 2006/ Accepted 23 October 2006

A multilaboratory collaborative study was performed in order to propose quality control limits for posaconazole disk diffusion susceptibility tests on Mueller-Hinton agar supplemented with 2% glucose and 0.5 µg of methylene blue per ml. Replicate tests were performed on three lots of prepared media using 5-µg posaconazole disks in each of eight laboratories to generate data to propose quality control zone diameter ranges for tests of Candida albicans ATCC 90028 (24 to 34 mm), C. parapsilosis ATCC 22019 (25 to 36 mm), C. tropicalis ATCC 750 (23 to 33 mm), and C. krusei ATCC 6258 (23 to 31 mm).

* Corresponding author. Mailing address: 9725 SW Commerce Circle, Suite A-1, Wilsonville, OR 97070. Phone: (503) 682-3232. Fax: (503) 682-2065. E-mail: SBrown{at}clinmicroinst.com.

{triangledown} Published ahead of print on 8 November 2006.

Journal of Clinical Microbiology, January 2007, p. 222-223, Vol. 45, No. 1
0095-1137/07/$08.00+0     doi:10.1128/JCM.01732-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Canton, E., Peman, J., Espinel-Ingroff, A., Martin-Mazuelos, E., Carrillo-Munoz, A., Martinez, J. P. (2008). Comparison of disc diffusion assay with the CLSI reference method (M27-A2) for testing in vitro posaconazole activity against common and uncommon yeasts. J Antimicrob Chemother 61: 135-138 [Abstract] [Full Text]  


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