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Journal of Clinical Microbiology, January 2007, p. 248-251, Vol. 45, No. 1
0095-1137/07/$08.00+0     doi:10.1128/JCM.01576-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Penile, Urethral, and Seminal Sampling for Diagnosis of Human Papillomavirus Infection in Men{triangledown}

Lucia Giovannelli,1* Maria Colomba Migliore,1 Giuseppina Capra,1 Maria Pia Caleca,1 Carmelina Bellavia,1 Antonio Perino,2 Enza Viviano,1 Domenica Matranga,3 and Pietro Ammatuna1

Dipartimento di Igiene e Microbiologia,1 Dipartimento di Ostetricia e Ginecologia,2 Dipartimento di Biotecnologie Mediche e Medicina Legale, Università di Palermo, Palermo, Italy3

Received 31 July 2006/ Returned for modification 6 October 2006/ Accepted 25 October 2006

Methods that used specimens from three genital sites (penile brushing [PB], urethral brushing [UB], and the retrieval of semen [SE]) from 50 men were examined for human papillomavirus (HPV) DNA detection. The rates of detection by PB, UB, SE, PB and UB, and PB and SE were 88.9%, 50.0%, 33.3%, 100%, and 97.2%, respectively. The use of PB and UB appears to be the most accurate method; as an alternative to UB, the use of SE with PB could be used to improve the rate of HPV DNA detection in men.

* Corresponding author. Mailing address: Dipartimento di Igiene e Microbiologia, Policlinico P. Giaccone, via del Vespro, 133 Palermo, Italy. Phone: 39 091 6553665. Fax: 39 091 6553676. E-mail: lucia.giov{at}unipa.it.

{triangledown} Published ahead of print on 8 November 2006.

Journal of Clinical Microbiology, January 2007, p. 248-251, Vol. 45, No. 1
0095-1137/07/$08.00+0     doi:10.1128/JCM.01576-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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