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Journal of Clinical Microbiology, January 2007, p. 252-254, Vol. 45, No. 1
0095-1137/07/$08.00+0     doi:10.1128/JCM.01489-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Newly Developed Primers for Comprehensive Amplification of the rpoB Gene and Detection of Rifampin Resistance in Mycobacterium tuberculosis{triangledown}

Leen Rigouts,1* Oscar Nolasco,2 Pim de Rijk,1 Elie Nduwamahoro,1 Armand Van Deun,1 Andy Ramsay,4 Jorge Arevalo,2,3 and Françoise Portaels1

Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium,1 Alexander von Humboldt Institute of Tropical Medicine, Universidad Peruana Cayetano Heredia, Lima, Peru,2 Departamento de Bioquímica, Biología Molecular y Farmacología, Facultad de Ciencias, Universidad Peruana Cayetano Heredia, Lima, Peru,3 UNICEF/UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR), World Health Organization, Geneva, Switzerland4

Received 19 July 2006/ Returned for modification 14 September 2006/ Accepted 24 October 2006

New rpoB gene primers for detecting Rifr in Mycobacterium tuberculosis complex bacteria achieved 100% specificity and 88% (fresh sputa) and 92% (ethanol-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rifr isolates examined, 97% had mutations within cluster I, 2% at codon 176, and 1% at codon 497.

* Corresponding author. Mailing address: Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat 155, 2000 Antwerpen, Belgium. Phone: 32 (0) 3 2476551. Fax: 32 (0) 3 2476333. E-mail: lrigouts{at}itg.be.

{triangledown} Published ahead of print on 8 November 2006.

Journal of Clinical Microbiology, January 2007, p. 252-254, Vol. 45, No. 1
0095-1137/07/$08.00+0     doi:10.1128/JCM.01489-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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