This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rigouts, L.
Right arrow Articles by Portaels, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rigouts, L.
Right arrow Articles by Portaels, F.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, January 2007, p. 252-254, Vol. 45, No. 1
0095-1137/07/$08.00+0     doi:10.1128/JCM.01489-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Newly Developed Primers for Comprehensive Amplification of the rpoB Gene and Detection of Rifampin Resistance in Mycobacterium tuberculosis{triangledown}

Leen Rigouts,1* Oscar Nolasco,2 Pim de Rijk,1 Elie Nduwamahoro,1 Armand Van Deun,1 Andy Ramsay,4 Jorge Arevalo,2,3 and Françoise Portaels1

Mycobacteriology Unit, Institute of Tropical Medicine, Antwerp, Belgium,1 Alexander von Humboldt Institute of Tropical Medicine, Universidad Peruana Cayetano Heredia, Lima, Peru,2 Departamento de Bioquímica, Biología Molecular y Farmacología, Facultad de Ciencias, Universidad Peruana Cayetano Heredia, Lima, Peru,3 UNICEF/UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR), World Health Organization, Geneva, Switzerland4

Received 19 July 2006/ Returned for modification 14 September 2006/ Accepted 24 October 2006

New rpoB gene primers for detecting Rifr in Mycobacterium tuberculosis complex bacteria achieved 100% specificity and 88% (fresh sputa) and 92% (ethanol-preserved sputa) diagnostic sensitivity and detected up to 4 CFU/sample. Of the 99 Rifr isolates examined, 97% had mutations within cluster I, 2% at codon 176, and 1% at codon 497.

* Corresponding author. Mailing address: Mycobacteriology Unit, Institute of Tropical Medicine, Nationalestraat 155, 2000 Antwerpen, Belgium. Phone: 32 (0) 3 2476551. Fax: 32 (0) 3 2476333. E-mail: lrigouts{at}itg.be.

{triangledown} Published ahead of print on 8 November 2006.

Journal of Clinical Microbiology, January 2007, p. 252-254, Vol. 45, No. 1
0095-1137/07/$08.00+0     doi:10.1128/JCM.01489-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Van Deun, A., Barrera, L., Bastian, I., Fattorini, L., Hoffmann, H., Kam, K. M., Rigouts, L., Rusch-Gerdes, S., Wright, A. (2009). Mycobacterium tuberculosis Strains with Highly Discordant Rifampin Susceptibility Test Results. J. Clin. Microbiol. 47: 3501-3506 [Abstract] [Full Text]  
  • Evans, J., Stead, M. C., Nicol, M. P., Segal, H. (2009). Rapid genotypic assays to identify drug-resistant Mycobacterium tuberculosis in South Africa. J Antimicrob Chemother 63: 11-16 [Abstract] [Full Text]  
  • Affolabi, D., Odoun, M., Sanoussi, N., Martin, A., Palomino, J. C., Kestens, L., Anagonou, S., Portaels, F. (2008). Rapid and Inexpensive Detection of Multidrug-Resistant Mycobacterium tuberculosis with the Nitrate Reductase Assay Using Liquid Medium and Direct Application to Sputum Samples. J. Clin. Microbiol. 46: 3243-3245 [Abstract] [Full Text]  
  • Chakravorty, S., Aladegbami, B., Motiwala, A. S., Dai, Y., Safi, H., Brimacombe, M., Helb, D., Alland, D. (2008). Rifampin Resistance, Beijing-W Clade-Single Nucleotide Polymorphism Cluster Group 2 Phylogeny, and the Rv2629 191-C Allele in Mycobacterium tuberculosis Strains. J. Clin. Microbiol. 46: 2555-2560 [Abstract] [Full Text]  
  • Quezada, C. M., Kamanzi, E., Mukamutara, J., De Rijk, P., Rigouts, L., Portaels, F., Amor, Y. B. (2007). Implementation Validation Performed in Rwanda To Determine Whether the INNO-LiPA Rif.TB Line Probe Assay Can Be Used for Detection of Multidrug-Resistant Mycobacterium tuberculosis in Low-Resource Countries. J. Clin. Microbiol. 45: 3111-3114 [Abstract] [Full Text]  
  • Affolabi, D., Odoun, M., Martin, A., Palomino, J. C., Anagonou, S., Portaels, F. (2007). Evaluation of Direct Detection of Mycobacterium tuberculosis Rifampin Resistance by a Nitrate Reductase Assay Applied to Sputum Samples in Cotonou, Benin. J. Clin. Microbiol. 45: 2123-2125 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»