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Journal of Clinical Microbiology, October 2007, p. 3151-3154, Vol. 45, No. 10
0095-1137/07/$08.00+0     doi:10.1128/JCM.02411-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Factors Influencing Broth Microdilution Antimicrobial Susceptibility Test Results for Dalbavancin, a New Glycopeptide Agent{triangledown}

Robert P. Rennie,1* Laura Koeth,2 Ronald N. Jones,3,4 Thomas R. Fritsche,3 Cindy C. Knapp,5 Scott B. Killian,5 and Beth P. Goldstein6

Medical Microbiology, University of Alberta Hospital, Edmonton, Alberta T6G 2J2, Canada,1 Laboratory Specialists, Inc., Westlake, Ohio 44145,2 JMI Laboratories, North Liberty, Iowa 52317,3 Tufts University School of Medicine, Boston, Massachusetts 02111,4 TREK Diagnostic Systems, Cleveland, Ohio 44131,5 Vicuron Pharmaceuticals, Inc., King of Prussia, Pennsylvania 194066

Received 30 November 2006/ Returned for modification 24 January 2007/ Accepted 24 July 2007

Performance of antimicrobial susceptibility tests with new agents requires careful consideration of the properties of the antimicrobial to ensure that the tests are standardized, reproducible, and reflect the true potency of the drug. Dalbavancin is a new glycopeptide with potent activity against gram-positive bacterial species. The investigations described here demonstrated that methodologic modifications of procedures are necessary to ensure consistent test results, both for quality control and for routine testing of clinical isolates. Dimethyl sulfoxide is the preferred primary solvent. The addition of 0.002% polysorbate-80 (a surfactant) to dalbavancin-containing wells in the reference broth microdilution assay resulted in consistent and reproducible MIC results for three quality control strains: Staphylococcus aureus ATCC 29213, Enterococcus faecalis ATCC 29212, and Streptococcus pneumoniae ATCC 49619. The same degree of consistency was observed among clinical isolates of gram-positive bacterial species tested in several clinical laboratories. These results indicate that the addition of 0.002% (final concentration) of the surfactant in broth microdilution tests produces optimal dalbavancin MICs required for accurate and reproducible clinical laboratory tests, without untoward influences of substrate binding or media constituents.

* Corresponding author. Mailing address: Microbiology and Public Health, University of Alberta Hospital, WMC 283.08, 8440 112 St., Edmonton, Alberta T6G 2J2, Canada. Phone: (780) 407-8806. Fax: (780) 407-8599. E-mail: robert.rennie{at}capitalhealth.ca

{triangledown} Published ahead of print on 1 August 2007.

Journal of Clinical Microbiology, October 2007, p. 3151-3154, Vol. 45, No. 10
0095-1137/07/$08.00+0     doi:10.1128/JCM.02411-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Biedenbach, D. J., Bell, J. M., Sader, H. S., Turnidge, J. D., Jones, R. N. (2009). Activities of Dalbavancin against a Worldwide Collection of 81,673 Gram-Positive Bacterial Isolates. Antimicrob. Agents Chemother. 53: 1260-1263 [Abstract] [Full Text]  
  • Arhin, F. F., Sarmiento, I., Belley, A., McKay, G. A., Draghi, D. C., Grover, P., Sahm, D. F., Parr, T. R. Jr., Moeck, G. (2008). Effect of Polysorbate 80 on Oritavancin Binding to Plastic Surfaces: Implications for Susceptibility Testing. Antimicrob. Agents Chemother. 52: 1597-1603 [Abstract] [Full Text]  


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