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Journal of Clinical Microbiology, October 2007, p. 3390-3392, Vol. 45, No. 10
0095-1137/07/$08.00+0     doi:10.1128/JCM.00505-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Multicenter Comparison of Molecular Methods for Detection of Legionella spp. in Sputum Samples{triangledown}

M. A. Bencini,1 A. J. C. van den Brule,2 E. C. J. Claas,3 M. H. A. Hermans,4 W. J. G. Melchers,5 G. T. Noordhoek,6 M. M. M. Salimans,7 J. Schirm,8 C. Vink,9,{dagger} A. van der Zee,10,{ddagger} and R. Jansen1*

Regional Public Health Laboratory Kennemerland, Boerhaavelaan 26, 2035 RC Haarlem, The Netherlands,1 Laboratory for Pathology and Medical Microbiology, PAMM Laboratories, Michelangelolaan 2, 5623 EJ Eindhoven, The Netherlands,2 Department of Medical Microbiology, Center of Infectious Diseases, Leiden University Medical Centre, P.O. Box 9600, 2300 RC Leiden, The Netherlands,3 Molecular Diagnostics, Jeroen Bosch Hospital, P.O. Box 90153, 5200 ME's-Hertogenbosch, The Netherlands,4 Department of Medical Microbiology, Radboud University Nijmegen Medical Center, P.O. Box 9101, 6500 HB Nijmegen, The Netherlands,5 Public Health Laboratory Friesland, P.O. Box 21020, 8900 JA Leeuwarden, The Netherlands,6 Department of Medical Microbiology and Immunology, Meander Medical Center, Utrechtseweg 160, 3818 ES Amersfoort, The Netherlands,7 Laboratory for Infectious Diseases, van Ketwich Verschuurlaan 92, 9721 SW Groningen, The Netherlands,8 Department of Medical Microbiology, Academic Hospital Maastricht, P.O. Box 5800, 6202 AZ Maastricht, The Netherlands,9 Laboratory of Medical Microbiology, St. Elisabeth Hospital, P.O. Box 747, 5000 AS Tilburg, The Netherlands,10

Received 6 March 2007/ Returned for modification 4 May 2007/ Accepted 21 July 2007

Legionellosis can be diagnosed by PCR using sputum samples. In this report, the methods of nine laboratories for 12 sputum samples with Legionella pneumophila and Legionella longbeachae are compared. We conclude that (i) liquefaction prevents PCR inhibition, (ii) the employed mip gene PCRs detected L. pneumophila only, and (iii) the 16S rRNA gene PCR detected both Legionella species and is preferred for the diagnosis of legionellosis.

* Corresponding author. Mailing address: Regional Public Health Laboratory Kennemerland, Boerhaavelaan 26, 2035 RC Haarlem, The Netherlands. Phone: 0031 23 530 7868. Fax: 0031 23 530 7805. E-mail: r.jansen{at}streeklabhaarlem.nl

{triangledown} Published ahead of print on 1 August 2007.

{dagger} Present address: Laboratory of Pediatrics, Erasmus Medical Center Rotterdam, P.O. Box 2040, 3000 CA Rotterdam, The Netherlands.

{ddagger} Present address: Laboratory for Molecular Diagnostics RLM/PAL, Albert Schweitzer Hospital, P.O. Box 899, 3300 AW Dordrecht, The Netherlands.

Journal of Clinical Microbiology, October 2007, p. 3390-3392, Vol. 45, No. 10
0095-1137/07/$08.00+0     doi:10.1128/JCM.00505-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kumpers, P., Tiede, A., Kirschner, P., Girke, J., Ganser, A., Peest, D. (2008). Legionnaires' disease in immunocompromised patients: a case report of Legionella longbeachae pneumonia and review of the literature. J Med Microbiol 57: 384-387 [Abstract] [Full Text]  


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