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Journal of Clinical Microbiology, October 2007, p. 3404-3407, Vol. 45, No. 10
0095-1137/07/$08.00+0     doi:10.1128/JCM.00458-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Comparison of Hand-Held Test Kits, Immunofluorescence Microscopy, Enzyme-Linked Immunosorbent Assay, and Flow Cytometric Analysis for Rapid Presumptive Identification of Yersinia pestis{triangledown}

H. Tomaso,1* P. Thullier,2 E. Seibold,1 V. Guglielmo,2 A. Buckendahl,1 L. Rahalison,3 H. Neubauer,4 H. C. Scholz,1 and W. D. Splettstoesser1

Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, 80937 Munich, Germany,1 Groupe de Biotechnologie des Anticorps, Départment de Biologie des Agents Transmissibles, Centre de Recherches du Service de Santé des Armées, 24 Avenue des Maquis du Grésivaudan, P.O. Box 87, 38702 La Tronche, France,2 World Health Organization Collaborating Center for Plague, Insitut Pasteur de Madagascar, Route de l'Institut Pasteur, Ambatofotsikely-Avaradoha, P.O. Box 1274, Antananarivo 101, Madagascar,3 Friedrich Loeffler Institut, Naumburgerstrasse 96a, 07743 Jena, Germany4

Received 28 February 2007/ Returned for modification 11 April 2007/ Accepted 17 July 2007

An in-house immunochromatographic test, Plague BioThreat Alert test strips, ABICAP columns, enzyme-linked immunosorbent assay, flow cytometry, and immunofluorescence microscopy were compared for the detection of the fraction 1 capsular antigen of Yersinia pestis, using spiked buffer and clinical specimens. Hand-held test kits proved to be excellent benchtop tools.

* Corresponding author. Mailing address: Bundeswehr Institute of Microbiology, Neuherbergstrasse 11, 80937 Munich, Germany. Phone: 49-8931683808. Fax: 49-8931683292. E-mail: herberttomaso{at}bundeswehr.org

{triangledown} Published ahead of print on 25 July 2007.

Journal of Clinical Microbiology, October 2007, p. 3404-3407, Vol. 45, No. 10
0095-1137/07/$08.00+0     doi:10.1128/JCM.00458-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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