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Journal of Clinical Microbiology, October 2007, p. 3411-3415, Vol. 45, No. 10
0095-1137/07/$08.00+0     doi:10.1128/JCM.01076-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Identification of Less-Common Streptococcus pneumoniae Serotypes by a Multiplex PCR-Based Reverse Line Blot Hybridization Assay{triangledown}

Fei Zhou,1 Fanrong Kong,1 Zhongsheng Tong,1,2 and Gwendolyn L. Gilbert1*

Centre for Infectious Diseases and Microbiology (CIDM), Institute of Clinical Pathology and Medical Research (ICPMR), Westmead, New South Wales, Australia,1 Research Laboratory for Infectious Skin Diseases, Department of Dermatology, Wuhan First Hospital, Wuhan 430022, People's Republic of China2

Received 26 May 2007/ Accepted 29 July 2007

We developed a multiplex PCR-based reverse line blot (mPCR/RLB) assay to identify 50 uncommon pneumococcal serotypes. In combination with a previously described mPCR/RLB assay (3), all 90 pneumococcal serotypes can be identified individually (32 serotypes) or, because of predictable cross-reactions, to within small groups of two to five related serotypes (58 serotypes), which can be distinguished using serotype-specific antisera.

* Corresponding author. Mailing address: Centre for Infectious Diseases and Microbiology (CIDM), Institute of Clinical Pathology and Medical Research (ICPMR), Westmead Hospital, Darcy Road, Westmead, New South Wales, 2145 Australia. Phone: (612) 9845 6255. Fax: (612) 9893 8659. E-mail: lyng{at}icpmr.wsahs.nsw.gov.au

{triangledown} Published ahead of print on 8 August 2007.

Journal of Clinical Microbiology, October 2007, p. 3411-3415, Vol. 45, No. 10
0095-1137/07/$08.00+0     doi:10.1128/JCM.01076-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.





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