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Journal of Clinical Microbiology, October 2007, p. 3421-3423, Vol. 45, No. 10
0095-1137/07/$08.00+0     doi:10.1128/JCM.00179-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Detection of Mutations Conferring Resistance to Linezolid in Enterococcus spp. by Fluorescence In Situ Hybridization{triangledown}

Guido Werner,2 Melanie Bartel,1 Nele Wellinghausen,1 Andreas Essig,1 Ingo Klare,2 Wolfgang Witte,2 and Sven Poppert1*

Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Ulm, Germany,1 Nosocomial Infections, Department of Infectious Diseases, Robert Koch Institute, Wernigerode, Germany2

Received 24 January 2007/ Returned for modification 8 March 2007/ Accepted 24 April 2007

A fluorescence in situ hybridization (FISH) assay was established to detect linezolid resistance (conferred by the mutation 2576G>T in the gene coding for the 23 string of the ribosomal RNA) in enterococci. The assay was evaluated with 106 Enterococcus isolates; it showed a sensitivity of 100% for the detection of phenotypic resistance and was even able to identify a single mutated allele in phenotypically linezolid-susceptible isolates.

* Corresponding author. Mailing address: Institute of Medical Microbiology and Hygiene, University Hospital of Ulm, Robert-Kochstr. 8, D-89081 Ulm, Germany. Phone: 49-731-50065334. Fax: 49-731-50065302. E-mail: sven.poppert{at}uniklinik-ulm.de

{triangledown} Published ahead of print on 2 July 2007.

Journal of Clinical Microbiology, October 2007, p. 3421-3423, Vol. 45, No. 10
0095-1137/07/$08.00+0     doi:10.1128/JCM.00179-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Scheetz, M. H., Knechtel, S. A., Malczynski, M., Postelnick, M. J., Qi, C. (2008). Increasing Incidence of Linezolid-Intermediate or -Resistant, Vancomycin-Resistant Enterococcus faecium Strains Parallels Increasing Linezolid Consumption. Antimicrob. Agents Chemother. 52: 2256-2259 [Abstract] [Full Text]  


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