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Journal of Clinical Microbiology, October 2007, p. 3427-3430, Vol. 45, No. 10
0095-1137/07/$08.00+0     doi:10.1128/JCM.01199-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Cryptococcus neoformans Typing by PCR Fingerprinting Using (GACA)4 Primers Based on C. neoformans Genome Project Data{triangledown}

Massimo Cogliati,1* Maria Carmela Esposto,1 Giordano Liberi,2 Anna Maria Tortorano,1 and Maria Anna Viviani1

Università degli Studi di Milano, Dipartimento di Sanità Pubblica-Microbiologia-Virologia, Sez. Sanità Pubblica, Lab. Micologia Medica, Milano, Italy,1 Fondazione Istituto FIRC di Oncologia Molecolare e Dipartimento di Scienze Biomediche e Biotecnologie, Università degli Studi di Milano, Via Adamello 16, 20139 Milano, Italy2

Received 14 June 2007/ Returned for modification 16 July 2007/ Accepted 26 July 2007

Four (GACA)4 PCR fingerprinting sequences, used as markers to identify serotypes A and D and AD hybrids, were retrieved in four Cryptococcus neoformans genome databases. Their locations, both in serotype A and D genomes, were confirmed by chromosomal hybridization with specific probes. Two sequences were recognized to code for hypothetical functional proteins.


* Corresponding author. Mailing address: Università degli Studi di Milano, Dipartimento di Sanità Pubblica-Microbiologia-Virologia, Sez. Sanità Pubblica, Lab. Micologia Medica, Via Pascal 36, 20133 Milano, Italy. Phone: 39 02 50315144-145. Fax: 39 02 50315146. E-mail: massimo.cogliati{at}unimi.it

{triangledown} Published ahead of print on 1 August 2007.


Journal of Clinical Microbiology, October 2007, p. 3427-3430, Vol. 45, No. 10
0095-1137/07/$08.00+0     doi:10.1128/JCM.01199-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.