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Journal of Clinical Microbiology, October 2007, p. 3427-3430, Vol. 45, No. 10
0095-1137/07/$08.00+0     doi:10.1128/JCM.01199-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Cryptococcus neoformans Typing by PCR Fingerprinting Using (GACA)₄ Primers Based on C. neoformans Genome Project Data

Università degli Studi di Milano, Dipartimento di Sanità Pubblica-Microbiologia-Virologia, Sez. Sanità Pubblica, Lab. Micologia Medica, Milano, Italy,¹ Fondazione Istituto FIRC di Oncologia Molecolare e Dipartimento di Scienze Biomediche e Biotecnologie, Università degli Studi di Milano, Via Adamello 16, 20139 Milano, Italy²

Received 14 June 2007/ Returned for modification 16 July 2007/ Accepted 26 July 2007

Four (GACA)₄ PCR fingerprinting sequences, used as markers to identify serotypes A and D and AD hybrids, were retrieved in four Cryptococcus neoformans genome databases. Their locations, both in serotype A and D genomes, were confirmed by chromosomal hybridization with specific probes. Two sequences were recognized to code for hypothetical functional proteins.

Published ahead of print on 1 August 2007.