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Journal of Clinical Microbiology, October 2007, p. 3439-3442, Vol. 45, No. 10
0095-1137/07/$08.00+0     doi:10.1128/JCM.01411-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Polymorphisms in gyrA and gyrB Genes among Mycobacterium avium subsp. paratuberculosis Type I, II, and III Isolates{triangledown}

E. Castellanos,1 A. Aranaz,1* B. Romero,1 L. de Juan,1 J. Alvarez,1 J. Bezos,1 S. Rodríguez,1 K. Stevenson,2 A. Mateos,1 and L. Domínguez1

Departamento de Sanidad Animal, Laboratorio de Vigilancia Sanitaria Veterinaria, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro s/n, 28040 Madrid, Spain,1 Moredun Research Institute, Pentlands Science Park, Penicuik EH26 OPZ, Scotland, United Kingdom2

Received 13 July 2007/ Accepted 22 July 2007

The analysis of the gyrA and gyrB genes of a panel of Mycobacterium avium subsp. paratuberculosis isolates from types I, II, and III detected type-specific single nucleotide polymorphisms. Based on these results, we developed a PCR and restriction enzyme analysis to discriminate type I and III isolates. The application of this technique would be the unique strategy to characterize these strains when there is not enough bacterial growth to perform pulsed-field gel electrophoresis and IS900 restriction fragment length polymorphism.

* Corresponding author. Mailing address: Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Avda. Puerta de Hierro s/n, 28040 Madrid, Spain. Phone: 34913943721. Fax: 34913943795. E-mail: address: alaranaz{at}vet.ucm.es

{triangledown} Published ahead of print on 1 August 2007.

Journal of Clinical Microbiology, October 2007, p. 3439-3442, Vol. 45, No. 10
0095-1137/07/$08.00+0     doi:10.1128/JCM.01411-07
Copyright © 2007, American Society for Microbiology. All Rights Reserved.



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